Human GRK3 Antibody

Catalog #: AF4785 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4785-100
AF4785-SP

Detection of Human GRK3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GRK3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GRK3 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GRK3
Pro469-Leu688
Accession # P35626

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

20 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GRK3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GRK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4785) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRK3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human GRK3 by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line, loaded at 0.2 mg/mL. A specific band was detected for GRK3 at approximately 80-81 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human GRK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4785) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRK3

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK3 (also known as beta -adrenergic receptor kinase 2, or beta ARK2) and GRK2 ( beta ARK1) are members of the beta -adrenergic receptor kinase subfamily. GRK3 may phosphorylate alpha -synuclein at S129, suggesting a potential involvement for this kinase in Parkinson’s disease.

Long Name

G protein-coupled Receptor Kinase 3

Entrez Gene IDs

157 (Human); 320129 (Mouse); 25372 (Rat)

Alternate Names

ADRBK2; adrenergic, beta, receptor kinase 2; BARK2; BARK2beta-adrenergic receptor kinase 2; Beta-ARK-2; EC 2.7.11; EC 2.7.11.15; G-protein-coupled receptor kinase 3; GRK3; GRK3Beta-ARK-2