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Human GPRC5C Antibody

Catalog #: MAB6594
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6594
MAB6594-SP
Detection of GPRC5C in PC-3 Human Cell Line by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPRC5C Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPRC5C in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 577315
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with recombinant human GPRC5C
Accession # Q9NQ84
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GPRC5C antibody in PC-3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of GPRC5C in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Human GPRC5C Monoclonal Antibody (Catalog # MAB6594, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPRC5C

G-protein coupled receptor family C group 5 member C (GPRC5C), also known as Retinoic acid induced gene 3 (RAIG-3), is an approximately 50 kDa protein with seven transmembrane segments and one site of potential N-linked glycosylation. It is predominantly expressed in the stomach, kidney, liver, pancreas, and prostate. Human GPRC5C shares 88% and 90% amino acid sequence identity with mouse and rat GPRC5C, respectively.

Long Name
G Protein-coupled Receptor, Family C, Group 5, Member C
Entrez Gene IDs
55890 (Human)
Alternate Names
G protein-coupled receptor, family C, group 5, member C; GPRC5C; MGC131820; RAIG3; RAIG-3G-protein coupled receptor family C group 5 member C; RAIG3orphan G-protein coupled receptor; retinoic acid responsive gene protein; Retinoic acid-induced gene 3 protein

Product Datasheets

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Citations for Human GPRC5C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Development and application of a high-content virion display human GPCR array
    Authors: Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al.
    Nature Communications
  2. Hyaluronic acid-GPRC5C signalling promotes dormancy in haematopoietic stem cells
    Authors: YW Zhang, J Mess, N Aizarani, P Mishra, C Johnson, MC Romero-Mul, J Rettkowski, K Schönberge, N Obier, K Jäcklein, NM Woessner, ME Lalioti, T Velasco-He, K Sikora, R Wäsch, B Lehnertz, G Sauvageau, T Manke, P Menendez, SG Walter, S Minguet, E Laurenti, S Günther, D Grün, N Cabezas-Wa
    Nature Cell Biology, 2022-06-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Proximity Ligation Assay

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