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Human GPR15 Antibody

Catalog #: MAB3654
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3654
MAB3654-SP
Detection of GPR15 in HEK293 Human Cell Line Transfected with human GRP15 and eGFP by Flow Cytometry.
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR15 Antibody Summary

Species Reactivity
Human
Specificity
Stains human GPR15-transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 367902
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR15
Met1-Leu360
Accession # P49685
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-reported
Seong, Y. et al. (2017) JCI Insight. 2(6): e90233. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GPR15 antibody in HEK293 Human Cell Line Transfected with human GRP15 and eGFP antibody by Flow Cytometry. View Larger

Detection of GPR15 in HEK293 Human Cell Line Transfected with human GRP15 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GRP15 and eGFP was stained with and either (A) Mouse Anti-Human GPR15 Monoclonal Antibody (Catalog # MAB3654) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR15

GPR15, also known as BOB, is a seven-transmembrane G protein-coupled receptor that is expressed in CD4+ T cells and alveolar macrophages. GPR15 functions as a cellular co-receptor for some isolates of HIV-1, HIV-2, and SIV through interactions with several viral envelope proteins. Human GPR15 shares 96%‑100% amino acid sequence identity with chimpanzee, macaque, and rhesus GPR15, and 76% with mouse GPR15.

Long Name
G Protein-coupled Receptor 15
Entrez Gene IDs
2838 (Human); 71223 (Mouse)
Alternate Names
Bob; Brother of Bonzo; G protein-coupled receptor 15; GPR15; G-protein coupled receptor 15; MGC126828; MGC126830

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Citations for Human GPR15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. GPR15 expressed in T lymphocytes from RA patients is involved in leukocyte chemotaxis to the synovium
    Authors: JC Fernández-, FL Ochoa-Gonz, M Zapata-Zúñ, E Mondragon-, EE Lara-Ramír, JL Ruíz-Carri, PA DelaCruz-F, E Layseca-Es, JA Enciso-Mor, JE Castañeda-
    Journal of leukocyte biology, 2022-09-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Tobacco-smoking induced GPR15-expressing T cells in blood do not indicate pulmonary damage
    Authors: M Bauer, B Fink, HJ Seyfarth, H Wirtz, A Frille
    BMC Pulm Med, 2017-11-28;17(1):159.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Trafficking receptor signatures define blood plasmablasts responding to tissue-specific immune challenge
    Authors: Y Seong, NH Lazarus, L Sutherland, A Habtezion, T Abramson, XS He, HB Greenberg, EC Butcher
    JCI Insight, 2017-03-23;2(6):e90233.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  4. CXCR6-mediated SIVagmSab Entry into Sabaeus African Green Monkey Lymphocytes Implicates Widespread Use of Non-CCR5 Pathways in Natural Host Infections
    Authors: Katherine S Wetzel
    J. Virol, 2017-01-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Role and species-specific expression of colon T cell homing receptor GPR15 in colitis.
    Authors: Nguyen L, Pan J, Dinh T, Hadeiba H, O'Hara E, Ebtikar A, Hertweck A, Gokmen M, Lord G, Jenner R, Butcher E, Habtezion A
    Nat Immunol, 2014-12-22;16(2):207-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis.
    Authors: Cartwright A, Schmutz C, Askari A, Kuiper J, Middleton J
    Cytokine, 2014-03-22;67(2):53-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Role of C-terminal membrane-proximal basic residues in cell surface trafficking of HIV coreceptor GPR15 protein.
    Authors: Okamoto, Yukari, Bernstein, Joshua D, Shikano, Sojin
    J Biol Chem, 2013-02-19;288(13):9189-99.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. The role of the alternative coreceptor GPR15 in SIV tropism for human cells.
    Authors: Kiene M, Marzi A, Urbanczyk A, Bertram S, Fisch T, Nehlmeier I, Gnirss K, Karsten C, Palesch D, Munch J, Chiodi F, Pohlmann S, Steffen I
    Virology, 2012-08-05;433(1):73-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Phosphorylation-dependent C-terminal Binding of 14-3-3 Proteins Promotes Cell Surface Expression of HIV Co-receptor GPR15.
    Authors: Okamoto Y, Shikano S
    J. Biol. Chem., 2010-12-28;286(9):7171-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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