Human Glycophorin A Biotinylated Antibody

Catalog #: BAM12281 Datasheet / COA / SDS

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BAM12281

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Human Glycophorin A Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glycophorin A, the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells [Greaves, M.F. et al. (1983) Blood 61(4):645].

Source

Monoclonal Mouse IgG₁ Clone # R10

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Abelson mouse leukemia virus-induced human pre-B tumor cells

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

TF‑1 human erythroleukemic cell line

Immunohistochemistry

Rearden, A. et al. (1985) Mol. Immunol. 22:369., San Miguel, J.F. et al. (1985) Br. J. Haematol. 59:297.

Immunoprecipitation

Blanchard, D. et al. (1983) Biochem. J. 213:399.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glycophorin A

Glycophorin A, also designated CD235a, is the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells (1).

References

Greaves, M.F. et al. (1983) Blood 61(4):645.

Entrez Gene IDs

2993 (Human)

Alternate Names

CD235a antigen; CD235a; glycophorin A (includes MN blood group); glycophorin A (MNS blood group); Glycophorin A; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GpMiIII; GPSAT; GYPA; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); Mi.V glycoprotein (24 AA); MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR