Human Glut5 Biotinylated Antibody

Catalog #: BAM1349 Datasheet / COA / SDS

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BAM1349

All Glut5 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glut5 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glut5. Stains human Glut5-transfected NS0 cells; also detects Glut5 on human microglia cells (Sasaki, A. et al. (2003) Neurosci. Lett. 338(1):17).

Source

Monoclonal Mouse IgG_2A Clone # 195205

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Glut5
Met1-Gln501
Accession # P22732

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

Human Glut5 transfected cells

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human small intestine and skeletal muscle

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut5

Glut5 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains, and isexpressed at variable levels in many tissues. Glut5 is expressed at highest levels insmall intestine and at lower levels in kidney, testis, skeletal muscle and adipose tissue (1, 2). Glut5 transports fructose in intestine, testis and other tissues.

Long Name

Glucose Transporter Type 5

Entrez Gene IDs

6518 (Human); 56485 (Mouse)

Alternate Names

Fructose transporter; Glucose transporter type 5, small intestine; Glut5; GLUT-5; GLUT5glucose transporter-like protein 5; SLC2A5; solute carrier family 2 (facilitated glucose transporter), member 5; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; solute carrier family 2, facilitated glucose transporter member 5