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Human Glut2 PE-conjugated Antibody

Catalog #: FAB1414P
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1414P
Detection of Glut2 in HepG2 Human Cell Line by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glut2 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes human Glut2 expression on human Glut2‑transfected NS0 cells, but not on control transfectants. Was shown to detect Glut2 on the surface of the human intestinal cell line Caco2 (2) in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with human Glut1 or human Glut3.
Source
Monoclonal Mouse IgG2A Clone # 199017
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Glut2
Met1-Val524
Accession # P11168
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Glut2 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Glut2 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut2 PE-conjugated Monoclonal Antibody (Catalog # FAB1414P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut2

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney, and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state (1).

References
  1. Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
  2. Mahraoui, L. et al. (1994) J. Biochem. 298:629.
Long Name
Glucose Transporter Type 2
Entrez Gene IDs
6514 (Human); 20526 (Mouse)
Alternate Names
GLUT2 Glucose transporter type 2, liver; Glut2; GLUT-2; SLC2A2; solute carrier family 2 (facilitated glucose transporter), member 2; solute carrier family 2, facilitated glucose transporter member 2

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Citations for Human Glut2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Fluorine-18 Fluorodeoxyglucose Uptake in Hepatocellular Carcinoma: Correlation with Glucose Transporters and p53 Expression
    Authors: Ana F. Brito, Ana M. Abrantes, Marina Ribeiro, Rui Oliveira, João Casalta-Lopes, Ana C. Gonçalves et al.
    Journal of Clinical and Experimental Hepatology
  2. Understanding the Role of GLUT2 in Dysglycemia Associated with Fanconi-Bickel Syndrome
    Authors: S Sharari, B Kabeer, I Mohammed, B Haris, I Pavlovski, I Hawari, AA Bhat, M Toufiq, S Tomei, R Mathew, N Syed, S Nisar, S Maacha, JC Grivel, D Chaussabel, J Ericsson, K Hussain
    Biomedicines, 2022-08-29;10(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Generation of Multipotent Stem Cells from Adult Human Peripheral Blood Following the Treatment with Platelet-Derived Mitochondria
    Authors: H Yu, W Hu, X Song, Y Zhao
    Cells, 2020-05-29;9(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Presence of diabetes autoantigens in extracellular vesicles derived from human islets
    Authors: CP Hasilo, S Negi, I Allaeys, N Cloutier, AK Rutman, M Gasparrini, É Bonneil, P Thibault, É Boilard, S Paraskevas
    Sci Rep, 2017-07-10;7(1):5000.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Mutations in SLC2A2 gene reveal hGLUT2 function in pancreatic beta cell development.
    Authors: Michau A, Guillemain G, Grosfeld A, Vuillaumier-Barrot S, Grand T, Keck M, L'Hoste S, Chateau D, Serradas P, Teulon J, De Lonlay P, Scharfmann R, Brot-Laroche E, Leturque A, Le Gall M
    J Biol Chem, 2013-08-28;288(43):31080-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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