Human Glucosamine (N-acetyl)-6‑Sulfatase/GNS Antibody

Catalog #: AF2484 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2484
AF2484-SP

All Glucosamine (N-acetyl)-6-Sulfatase/GNS Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glucosamine (N-acetyl)-6‑Sulfatase/GNS Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glucosamine (N-acetyl)-6‑Sulfatase/GNS in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glucosamine (N-acetyl)‑6-Sulfatase/GNS
Val37-Leu552
Accession # P15586

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Glucosamine (N-acetyl)‑6-Sulfatase/GNS (Catalog # 2484-SUC)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Glucosamine (N-acetyl)‑
6-Sulfatase/GNS (Catalog # 2484-SUC), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glucosamine (N-acetyl)-6-Sulfatase/GNS

A member of the sulfatase family, GNS is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and keratan sulfate (1, 2). It hydrolyzes the 6-sulfate group of the N-acetyl-D-glucosamine 6-sulfate units of the GAG. GNS deficiency results in mucopolysaccharidosis type IIID (MPS IIID or Sanfilippo D Syndrome), an inborn error leading to lysosomal accumulation of heparan sulfate. MPS IIID has profound mental deterioration, hyperactivity, and relatively mild somatic manifestations. The deduced amino acid sequence of human GNS consists of a signal peptide (residues 1‑36) and a mature chain (residues 37‑552) that may be further processed into N-terminal and C-terminal fragments (3). Recombinant human GNS corresponds to the single chain and has sulfatase activity.

References

Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
Neufeld, E.F. and J. Muenzer (2001) in The Metabolic and Molecular Basis of Inherited Disease, Scriver, C.R. et al. (eds.) pp. 3421 - 3452, New York, McGraw-Hill.
Robertson, D.A. et al. (1992) Biochem. J. 288:539.

Entrez Gene IDs

2799 (Human)

Alternate Names

EC 3.1.6; EC 3.1.6.14; G6Sglucosamine-6-sulfatase; glucosamine (N-acetyl)-6-sulfatase; Glucosamine6Sulfatase; Glucosamine-6-Sulfatase; GNS; MGC21274; N-acetylglucosamine-6-sulfatase