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Human GITR/TNFRSF18 Fluorescein-conjugated Antibody

Catalog #: FAB689F
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB689F-100
FAB689F-025
Detection of GITR/TNFRSF18 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GITR/TNFRSF18 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse (rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
Source
Monoclonal Mouse IgG1 Clone # 110416
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18
Gln26-Glu161 (Thr43Ala)
Accession # Q9Y5U5
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GITR/TNFRSF18 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of GITR/TNFRSF18 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 1 µg/mL PHA for 24 hours were stained with Mouse Anti-Human GITR/TNFRSF18 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB689F, filled histogram) or isotype control antibody (IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of GITR/TNFRSF18 in RPMI8226 (positive cell line)/Jurkat (negative cell line) by Flow Cytometry. RPMI8226 (positive cell line)/Jurkat (negative cell line) were stained with Mouse Anti-Human GITR/TNFRSF18 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB689F, filled histogram) or, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up‑regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

References
  1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
  2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
  3. Gurney, A.L. et al. (1999) Current Biology 9:215.
  4. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.
Long Name
Glucocorticoid Induced TNF Receptor Family Related Gene
Entrez Gene IDs
8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
Alternate Names
Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18

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Citations for Human GITR/TNFRSF18 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Stem cell source-dependent reconstitution of FOXP3+ T cells after pediatric SCT and the association with allo-reactive disease.
    Authors: Reubsaet, L L, de Pagter, A P J, van Baarle, D, Keukens, L, Nanlohy, N, Sanders, E A M, Prakken, B J, Boelens, J J, de Kleer, I M
    Bone Marrow Transplant, 2012-09-24;48(4):502-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Expression of the T cell receptor alpha/beta on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
    Authors: Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
    PLoS ONE, 2011-01-11;6(1):e15997.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Generation and accumulation of immunosuppressive adenosine by human CD4+CD25highFOXP3+ regulatory T cells.
    Authors: Mandapathil M, Hilldorfer B, Szczepanski MJ, Czystowska M, Szajnik M, Ren J, Lang S, Jackson EK, Gorelik E, Whiteside TL
    J. Biol. Chem., 2009-10-26;285(10):7176-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. CD4+CD25high T cell numbers are enriched in the peripheral blood of patients with rheumatoid arthritis.
    Authors: Han GM, O'Neil-Andersen NJ, Zurier RB, Lawrence DA
    Cell. Immunol., 2008-07-22;253(1):92-101.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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