Human GHSR Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8370R-100UG
Detection of GHSR in HEK293 Human Cell Line Transfected with Human GHSR and eGFP by Flow Cytometry.
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Human GHSR Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects GHSR on transfectants by flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # 502430
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GHSR
Accession # NP_940799
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GHSR antibody in HEK293 Human Cell Line Transfected with Human GHSR and eGFP antibody by Flow Cytometry. View Larger

Detection of GHSR in HEK293 Human Cell Line Transfected with Human GHSR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GHSR or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GHSR Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB8370R). Gates were set based on Mouse IgG1 Isotype Control staining (Catalog # IC002R, data not shown). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GHSR

GHSR is a member of the G protein-coupled receptor family that may play a role in energy homeostasis and regulation of body weight. Two conserved transcript variants for GHSR are expressed in several tissues. This transcript, 1a, excises an intron and encodes the functional protein. GHSR is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. Mutations in GHSR are associated with autosomal idiopathic short stature.

Long Name
Growth Hormone Secretagogue Receptor
Entrez Gene IDs
2693 (Human); 208188 (Mouse); 84022 (Rat)
Alternate Names
GH-releasing peptide receptor; Ghrelin receptor; GHRP; GHSR; GHS-R; growth hormone secretagogue receptor type 1; growth hormone secretagogue receptor

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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