Human GFI-1 Antibody Summary
Pro2-Leu250
Accession # Q99684
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of GFI-1 antibody in THP-1 Human Cell Line antibody by Flow Cytometry. Detection of GFI-1 antibody in THP-1 Human Cell Line antibody by Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/GFI1_AF3540_Flow_Cytometry_23623.jpg)
Detection of GFI-1 in THP-1 Human Cell Line by Flow Cytometry. THP-1 monocytic leukemia cell line was stained with Goat Anti-Human GFI-1SOX2 Affinity Purified Polyclonal Antibody (Catalog # AF3540, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by APC-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
![Detection of Human GFI-1 by Western Blot Detection of Human GFI-1 by Western Blot](https://resources.rndsystems.com/images/datasheets/af3540_human-gfi-1-affinity-purified-polyclonal-ab-41202412394973.jpg)
Detection of Human GFI-1 by Western Blot GFI1 interacts with proteins involved in the DDR. a GFI1-Flag fusion protein was immunoprecipitated from 293T cells. Co-precipitated proteins were run on polyacrylamide gel and stained with Coomassie blue. b Peptide sequence of MRE11 with peptides identified through mass spectrometry highlighted and corresponding spectra below. c Peptide sequence of PRMT1 with peptides identified through mass spectrometry highlighted and corresponding spectra below. d Variants of the GFI1-Flag fusion protein were immunoprecipitated from 293T cells. Extracts were separated by SDS–PAGE and blotted for the indicated proteins. e Diagram of the different GFI1 fusion proteins used in d and g. f GFI1-Flag fusion protein was immunoprecipitated in 293T cells in the presence or absence of benzonase. Extracts were separated by SDS–PAGE and blotted for MRE11. g GFI1 KO Jurkat cells were electroporated with plasmids expressing GFI1 variant constructs as shown in e. GFI1 KO and parental control Jurkat cells were used as controls. After 24 h, cells were exposed to 5 Gy IR and allowed to recover for the indicated time. Cells were lysed and analyzed by alkaline Comet assay. Comet tail moment averages are shown. One of three replicate experiments is shown. Error bars represent s.d. h Endogenous GFI1 protein was immunoprecipitated in SupT1 cells. Co-precipitated proteins were separated by SDS–PAGE and blotted for MRE11 and PRMT1. i Endogenous GFI1 protein was immunoprecipitated in Jurkat cells. Co-precipitated proteins were separated by SDS–PAGE and blotted for MRE11 and PRMT1 Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03817-5), licensed under a CC-BY license. Not internally tested by R&D Systems.
![Detection of Human GFI-1 by Western Blot Detection of Human GFI-1 by Western Blot](https://resources.rndsystems.com/images/datasheets/af3540_human-gfi-1-affinity-purified-polyclonal-ab-41202412401266.jpg)
Detection of Human GFI-1 by Western Blot GFI1 activities are independent of DNA damage. a GFI1-Flag fusion protein was immunoprecipitated in 293T cells treated with 5 Gy IR and allowed to recover for the indicated amount of time. Extracts were separated by SDS–PAGE and blotted for the indicated proteins. b SupT1 cells were spread on glass slides 15 min and 1 h after irradiation using a Cytospin, stained for endogenous Gfi1 and gamma -H2AX and visualized for immunofluorescence by confocal microscopy. Control cells stained without primary antibody but with secondary antibodies are shown. c U2OS cells carrying a LacO array and expressing a LacR-Fok1-mCherry endonuclease were transfected with a vector expressing the GFI1-GFP fusion protein. These cells were plated on cover glass, stained for gamma -H2AX and visualized for immunofluorescence by confocal microscopy. d U2OS cells expressing a GFI1-GFP fusion protein were exposed to 405 nm UV micro-irradiation and the recruitment of the GFI1-GFP fusion protein to the site of damage was quantified by confocal microscopy. Average signal intensity is shown with error bars representing s.d. Recruitment of Ku80-mRuby2 fusion protein and GFP protein are shown as controls. Representative images of selected time points are shown on the right. Scale bar represents 10 μm Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03817-5), licensed under a CC-BY license. Not internally tested by R&D Systems.
![Detection of Human Human GFI-1 Antibody by Immunoprecipitation Detection of Human Human GFI-1 Antibody by Immunoprecipitation](https://resources.rndsystems.com/images/datasheets/af3540_human-gfi-1-affinity-purified-polyclonal-ab-22820241721163.jpg)
Detection of Human Human GFI-1 Antibody by Immunoprecipitation GFI1 interacts with proteins involved in the DDR. a GFI1-Flag fusion protein was immunoprecipitated from 293T cells. Co-precipitated proteins were run on polyacrylamide gel and stained with Coomassie blue. b Peptide sequence of MRE11 with peptides identified through mass spectrometry highlighted and corresponding spectra below. c Peptide sequence of PRMT1 with peptides identified through mass spectrometry highlighted and corresponding spectra below. d Variants of the GFI1-Flag fusion protein were immunoprecipitated from 293T cells. Extracts were separated by SDS–PAGE and blotted for the indicated proteins. e Diagram of the different GFI1 fusion proteins used in d and g. f GFI1-Flag fusion protein was immunoprecipitated in 293T cells in the presence or absence of benzonase. Extracts were separated by SDS–PAGE and blotted for MRE11. g GFI1 KO Jurkat cells were electroporated with plasmids expressing GFI1 variant constructs as shown in e. GFI1 KO and parental control Jurkat cells were used as controls. After 24 h, cells were exposed to 5 Gy IR and allowed to recover for the indicated time. Cells were lysed and analyzed by alkaline Comet assay. Comet tail moment averages are shown. One of three replicate experiments is shown. Error bars represent s.d. h Endogenous GFI1 protein was immunoprecipitated in SupT1 cells. Co-precipitated proteins were separated by SDS–PAGE and blotted for MRE11 and PRMT1. i Endogenous GFI1 protein was immunoprecipitated in Jurkat cells. Co-precipitated proteins were separated by SDS–PAGE and blotted for MRE11 and PRMT1 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29651020), licensed under a CC-BY license. Not internally tested by R&D Systems.
![Detection of Human Human GFI-1 Antibody by Immunoprecipitation Detection of Human Human GFI-1 Antibody by Immunoprecipitation](https://resources.rndsystems.com/images/datasheets/af3540_human-gfi-1-affinity-purified-polyclonal-ab-22820241721195.jpg)
Detection of Human Human GFI-1 Antibody by Immunoprecipitation GFI1 interacts with proteins involved in the DDR. a GFI1-Flag fusion protein was immunoprecipitated from 293T cells. Co-precipitated proteins were run on polyacrylamide gel and stained with Coomassie blue. b Peptide sequence of MRE11 with peptides identified through mass spectrometry highlighted and corresponding spectra below. c Peptide sequence of PRMT1 with peptides identified through mass spectrometry highlighted and corresponding spectra below. d Variants of the GFI1-Flag fusion protein were immunoprecipitated from 293T cells. Extracts were separated by SDS–PAGE and blotted for the indicated proteins. e Diagram of the different GFI1 fusion proteins used in d and g. f GFI1-Flag fusion protein was immunoprecipitated in 293T cells in the presence or absence of benzonase. Extracts were separated by SDS–PAGE and blotted for MRE11. g GFI1 KO Jurkat cells were electroporated with plasmids expressing GFI1 variant constructs as shown in e. GFI1 KO and parental control Jurkat cells were used as controls. After 24 h, cells were exposed to 5 Gy IR and allowed to recover for the indicated time. Cells were lysed and analyzed by alkaline Comet assay. Comet tail moment averages are shown. One of three replicate experiments is shown. Error bars represent s.d. h Endogenous GFI1 protein was immunoprecipitated in SupT1 cells. Co-precipitated proteins were separated by SDS–PAGE and blotted for MRE11 and PRMT1. i Endogenous GFI1 protein was immunoprecipitated in Jurkat cells. Co-precipitated proteins were separated by SDS–PAGE and blotted for MRE11 and PRMT1 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29651020), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFI-1
Human GFI-1 is a 55 kDa, 422 amino acid (aa) nuclear, zinc-finger transcriptional regulator. It contains an N-terminal SNAG domain, an Ala/Gly-rich region, and a C‑terminus with six C2H2-type zinc finger motifs. GFI-1 binds DNA in a sequence-specific manner. GFI-1 functions as a transcription repressor in lymphoid cells and is required for neutrophil maturation. It also regulates self-renewal and is essential for the functional integrity of hematopoietic stem cells. Over the region used for immunization, human GFI-1 shares less than 50% amino acid sequence homology with human GFI-1B. It also shares 79% and 89% aa sequence identity with mouse and canine GFI-1, respectively.
Product Datasheets
Citations for Human GFI-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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GFI1 tethers the NuRD complex to open and transcriptionally active chromatin in myeloid progenitors
Authors: Anne Helness, Jennifer Fraszczak, Charles Joly-Beauparlant, Halil Bagci, Christian Trahan, Kaifee Arman et al.
Communications Biology
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Statin therapy is associated with lower prevalence of gut microbiota dysbiosis
Authors: S Vieira-Sil, G Falony, E Belda, T Nielsen, J Aron-Wisne, R Chakaroun, SK Forslund, K Assmann, M Valles-Col, TTD Nguyen, S Proost, E Prifti, V Tremaroli, N Pons, E Le Chateli, F Andreelli, JP Bastard, LP Coelho, N Galleron, TH Hansen, JS Hulot, C Lewinter, HK Pedersen, B Quinquis, C Rouault, H Roume, JE Salem, NB Søndertoft, S Touch, ME Dumas, SD Ehrlich, P Galan, JP Gøtze, T Hansen, JJ Holst, L Køber, I Letunic, J Nielsen, JM Oppert, M Stumvoll, H Vestergaar, JD Zucker, P Bork, O Pedersen, F Bäckhed, K Clément, J Raes
Nature, 2020-05-06;581(7808):310-315.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Transcription factor induced conversion of human fibroblasts towards the hair cell lineage
Authors: MB Duran Alon, I Lopez Hern, MA de la Fuen, J Garcia-San, F Giraldez, T Schimmang
PLoS ONE, 2018-07-06;13(7):e0200210.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Single-cell analysis of mixed-lineage states leading to a binary cell fate choice.
Authors: Olsson A, Venkatasubramanian M, Chaudhri V, Aronow B, Salomonis N, Singh H, Grimes H
Nature, 2016-08-31;537(7622):698-702.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Threshold Levels of Gfi1 Maintain E2A Activity for B Cell Commitment via Repression of Id1
PLoS ONE, 2016-07-28;11(7):e0160344.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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