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Human GDF-11/GDF-8 Antibody

Catalog #: MAB19582 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB19582
MAB19582-SP
Induction of Hemoglobin Expression by GDF‑11/BMP‑11 and Neutralization by anti-GDF‑11/GDF‑8 Antibody.
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Human GDF-11/GDF-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GDF-11/BMP-11 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with mouse GDF-8 is observed.
Source
Monoclonal Mouse IgG1 Clone # 743835
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GDF-11/BMP-11
Asn299-Ser407
Accession # O95390
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human GDF‑11/BMP‑11 Monoclonal Antibody (Catalog # MAB19584).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human GDF-11/BMP-11 DuoSet ELISA Kit (Catalog # DY1958-05) for convenient development of a sandwich ELISA.

 
Neutralization
Measured by its ability to neutralize GDF‑11/BMP‑11 induction of hemoglobin expression in the K562 human chronic myelogenous leukemia cell line, Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The Neutralization Dose (ND50) is typically 0.04-0.2 μg/mL in the presence of 25 ng/mL Recombinant Human GDF‑11/BMP‑11. This antibody also neutralizes GDF-8 in a similar K562 bioassay.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Induction of Hemoglobin Expression by GDF‑11/BMP‑11 and Neutralization by anti-GDF‑11/GDF‑8 Antibody. View Larger

Induction of Hemoglobin Expression by GDF‑11/BMP‑11 and Neutralization by anti-GDF‑11/GDF‑8 Antibody. Recombinant Human GDF-11/BMP-11 (Catalog # 1958-GD) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the pseudoperoxidase assay. Induction of hemoglobin expression by GDF-11/BMP-11 (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582). The ND50 is typically 0.04-0.2 µg/mL. This antibody also neutralizes GDF-8 in a similar K562 bioassay.

ELISA Human GDF-11/BMP-11 Antibody in ELISA Standard Curve. View Larger

Human GDF‑11/BMP‑11 ELISA Standard Curve. Recombinant Human GDF-11/BMP-11 protein was serially diluted 2-fold and captured by Mouse Anti-Human GDF-11/BMP-11 Monoclonal Antibody (Catalog # MAB19584) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GDF-11/GDF-8 Monoclonal Antibody (Catalog # MAB19582) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-11/GDF-8

Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid (aa) prepropeptide processed into a 109 aa mature protein. Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. At the amino acid sequence level, mature human, mouse, rat and chicken GDF-11 are 99-100% identical. Mature GDF-11 and GDF-8 share 90% amino acid sequence identity. GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain (1). GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning (2). Systemic GDF-11 levels decline with age and administration of higher levels of GDF-11 can reverse age-related cardiac hypertrophy (3). In addition, systemic administration of recombinant GDF-11 protein restores genomic integrity and health of muscle stem cells, neurovasculature and enhances neurogenesis (4, 5).

References
  1. Nakashima, M. et al. (1999) Mech. Dev. 80:185.  
  2. Oh, S.P. et al. (2002) Genes & Dev. 16:274.
  3. Loffredo, F.S. et. al. (2013) Cell. 153.828.
  4. Katsimpardi, L. et. al. (2014) Science. 344.630.
  5. Sinha, M. et. al. (2014) Science. 344.649.
Long Name
Growth Differentiation Factor 11/8
Entrez Gene IDs
10220 (Human)
Alternate Names
BMP11; BMP-11; bone morphogenetic protein 11; GDF-11; GDF-11/GDF-8; growth/differentiation factor 11; VHO

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