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Human GATA-6 Biotinylated Antibody

Catalog #: BAF1700 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF1700
GATA‑6 in KATO-III Human Cell Line.
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Human GATA-6 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GATA-6.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GATA-6
Met1-Ala449
Accession # Q92908-2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GATA‑6
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry GATA‑6 antibody in KATO-III Human Cell Line by Immunocytochemistry (ICC). View Larger

GATA‑6 in KATO-III Human Cell Line. GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Human GATA‑6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red, upper panel; Catalog # NL999) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-6

GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

Entrez Gene IDs
2627 (Human)
Alternate Names
GATA binding protein 6; GATA6; GATA-6; GATA-binding factor 6; GATA-binding protein 6; transcription factor GATA-6

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