Human GATA-5 Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human GATA‑5 antibody by Western Blot. Detection of Human GATA‑5 antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/GATA-5_AF2170_Western_Blot_5177.jpg)
Detection of Human GATA‑5 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2170) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA-5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![Detection of GATA-5-regulated Genes antibody by Chromatin Immunoprecipitation. Detection of GATA-5-regulated Genes antibody by Chromatin Immunoprecipitation.](https://resources.rndsystems.com/images/datasheets/antibody/GATA-5_AF2170_Chromatin_Immunoprecipitation_11226.jpg)
Detection of GATA‑5-regulated Genes by Chromatin Immunoprecipitation. HeLa human cervical epithelial carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA-5/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human GATA-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2170) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Themucin4promoter was detected by standard PCR.
![GATA-5 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). GATA-5 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).](https://resources.rndsystems.com/images/datasheets/antibody/GATA5_AF2170_Immunocytochemistry__Immunofluorescence_18851.jpg)
GATA‑5 in HepG2 Human Cell Line. GATA-5 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human GATA-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2170) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Preparation and Storage
Background: GATA-5
GATA-5 belongs to the GATA family of transcription factors which bind to the consensus DNA sequence (AIT) GATA (AIG) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA5 is important in the development and differentiation of endoderm-derived organs, including heart and gastrointestinal tissues. GATA-5 has also been implicated in cancer development (1, 2).
Product Datasheets
Citation for Human GATA-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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The human mucin MUC4 is transcriptionally regulated by caudal-related homeobox, hepatocyte nuclear factors, forkhead box A, and GATA endodermal transcription factors in epithelial cancer cells.
Authors: Jonckheere N, Vincent A, Perrais M, Ducourouble MP, Male AK, Aubert JP, Pigny P, Carraway KL, Freund JN, Renes IB, Van Seuningen I
J. Biol. Chem., 2007-06-06;282(31):22638-50.
Species: Human
Sample Types: Cell Lysates
Applications: ChIP
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