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Human GATA-4 Biotinylated Antibody

Catalog #: BAF2606
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF2606
GATA‑4 in iBJ6 iPS Cell Line.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GATA-4 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GATA‑4 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human GATA-1, -2, -3, -5, and -6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GATA-4
Met27-Phe211
Accession # P43694
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GATA‑4
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry GATA-4 antibody in iBJ6 iPS Cell Line by Immunocytochemistry (ICC). View Larger

GATA‑4 in iBJ6 iPS Cell Line. GATA-4 was detected in immersion fixed iBJ6, an induced pluripotent stem cell line, differentiated to endoderm using Goat Anti-Human GATA-4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2606) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-4

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Entrez Gene IDs
2626 (Human)
Alternate Names
GATA binding protein 4; GATA4; GATA-4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; transcription factor GATA-4

Product Datasheets

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Citations for Human GATA-4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Development of Human Adrenocortical Adenoma (HAA1) Cell Line from Zona Reticularis
    Authors: HK Ghayee, Y Xu, H Hatch, R Brockway, AS Multani, T Gu, WB Bollag, A Turcu, WE Rainey, J Rege, K Nanba, VJ Bhagwandin, F Nwariaku, V Stastny, AF Gazdar, JW Shay, RJ Auchus, SG Tevosian
    International Journal of Molecular Sciences, 2022-12-29;24(1):.
    Species: Human
    Sample Types: Whole Cell
    Applications: ICC
  2. Lumen Expansion Facilitates Epiblast-Primitive Endoderm Fate Specification during Mouse Blastocyst Formation
    Authors: Allyson Quinn Ryan, Chii Jou Chan, François Graner, Takashi Hiiragi
    Developmental Cell
  3. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.
    Authors: Drukker M, Tang C, Ardehali R
    Nat. Biotechnol., 2012-05-27;30(6):531-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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