Human Fyn Antibody

Catalog #: AF3574 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3574
AF3574-SP

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Human Fyn Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fyn in direct ELISAs.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Fyn
Met1-Gly83
Accession # P06241

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

10-20 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Fyn in MOLT‑4 Human Cell Line. Fyn was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Goat Anti-Human Fyn Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3574) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fyn

Fyn is a ubiquitously expressed member of the Src family of intracellular tyrosine kinases. Fyn activity is implicated in T cell receptor signaling, adhesion-mediated signaling, and regulation of brain function. Mice lacking fyn display various deficits, including impaired spatial memory, hypomyelination, and uncoordinated hippocampal structure, suggesting that Fyn is involved in multiple neural signaling pathways.

Long Name

FYN oncogene related to SRC, FGR, YES

Entrez Gene IDs

2534 (Human)

Alternate Names

EC 2.7.10; EC 2.7.10.2; FYN oncogene related to SRC, FGR, YES; Fyn; MGC45350; OKT3-induced calcium influx regulator; p59-Fyn; protein-tyrosine kinase fyn; Proto-oncogene c-Fyn; Proto-oncogene Syn; proto-oncogene tyrosine-protein kinase fyn; SLK; SLKc-syn protooncogene; src/yes-related novel; Src-like kinase; SYN; tyrosine kinase p59fyn(T); tyrosine-protein kinase Fyn