Human FPRL2/FPR3 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3896P-100
Detection of FPRL2/FPR3 in HEK293 Human Cell Line Transfected with Human FPRL2/FPR3 and eGFP by Flow Cytometry.
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Human FPRL2/FPR3 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human FPRL2. Stains human FPRL2 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 374822
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human FPRL2
Met1-Met352
Accession # P25089
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of FPRL2/FPR3 antibody in HEK293 Human Cell Line Transfected with Human FPRL2/FPR3 and eGFP antibody by Flow Cytometry. View Larger

Detection of FPRL2/FPR3 in HEK293 Human Cell Line Transfected with Human FPRL2/FPR3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) FPRL2/FPR3 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human FPRL2/FPR3 PE-conjugated Monoclonal Antibody (Catalog # FAB3896P). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FPRL2/FPR3

FPRL2, also known as FMLP receptor-like 2, is a 40 kDa 7TM protein that is expressed on monocytes and dendritic cells. It interacts with formyl and acetyl peptides to attract these cells to sites of infection. FPRL2 signaling also inhibits DC maturation. Human FPRL2 shares 63% and 62% amino acid sequence identity with mouse and rat FPRL2, respectively.

Long Name
Formyl Peptide Receptor-like 2
Entrez Gene IDs
2359 (Human); 14294 (Mouse)
Alternate Names
FMLP-R-II; FMLPY; formyl peptide receptor 3; Formyl peptide receptor-like 2FMLP-related receptor II; FPR3; FPRH1; FPRH1FPRH2; FPRH2; FPRL2; FPRL2FML2_HUMAN; N-formyl peptide receptor 3; RMLP-R-I

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