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Human FoxF2 Antibody

Catalog #: AF6988
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6988
AF6988-SP
Detection of Human FoxF2 by Western Blot.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FoxF2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FoxF2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) FoxF1, rhFoxP3, and rhFoxC2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FoxF2
Ala324-Met444
Accession # Q12947
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FoxF2 antibody by Western Blot. View Larger

Detection of Human FoxF2 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FoxF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6988) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxF2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxF2

FoxF2 (Forkhead box protein F2; also FREAC-2 and FKHL6) is a 42-46 kDa member of the winged helix transcription factor gene family. It is a transcriptional activator that is expressed in embryonic mesenchyme, inducing the synthesis if BMP4 and ECM. In the adult, it is expressed in placenta and lung, specifically in type II pneumocytes plus bronchiolar epithelium, inducing the synthesis of C10 and surfactant proteins B and C. Human FoxF2 is 444 amino acids (aa) in length. It contains one poly-Ala repeat (aa 33-41), a poly-Ser region (aa 46-67), and a poly-Gly segment (aa 77-84), followed by a forkhead DNA binding domain (aa 99-190) and three homopolymeric "runs" involving His (aa 262-272), Gly (aa 301-306) and Ser (aa 312-315). Over aa 324-444, human FoxF2 shares 99% aa identity with mouse FoxF2.

Long Name
Forkhead Box F2
Entrez Gene IDs
2295 (Human); 14238 (Mouse)
Alternate Names
FKHL6; FKHL6forkhead box protein F2; forkhead box F2; forkhead-like 6; Forkhead-related activator 2; Forkhead-related protein FKHL6; Forkhead-related transcription factor 2; FoxF2; FREAC2; FREAC2FREAC-2

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Citations for Human FoxF2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Neural crest cells require Meis2 for patterning the mandibular arch via the Sonic hedgehog pathway
    Authors: Jaroslav Fabik, Katarina Kovacova, Zbynek Kozmik, Ondrej Machon
    Biology Open
  2. Stromal FOXF2 suppresses prostate cancer progression and metastasis by enhancing antitumor immunity
    Authors: D Jia, Z Zhou, OJ Kwon, L Zhang, X Wei, Y Zhang, M Yi, MP Roudier, MC Regier, R Dumpit, PS Nelson, M Headley, L True, DW Lin, C Morrissey, CJ Creighton, L Xin
    Nature Communications, 2022-11-11;13(1):6828.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Hedgehog signaling patterns the oral-aboral axis of the mandibular arch
    Authors: J Xu, H Liu, Y Lan, M Adam, DE Clouthier, S Potter, R Jiang
    Elife, 2019-01-14;8(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Gastroparesis is associated with decreased FOXF1 and FOXF2 in humans, and loss of FOXF1 and FOXF2 results in gastroparesis in mice
    Authors: Herring BP, Hoggatt AM, Gupta A, Wo JM.
    Neurogastroenterol. Motil.
  5. Unbiased identification of novel transcription factors in striatal compartmentation and striosome maturation
    Authors: Maria-Daniela Cirnaru, Sicheng Song, Kizito-Tshitoko Tshilenge, Chuhyon Corwin, Justyna Mleczko, Carlos Galicia Aguirre et al.
    eLife

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