Human FoxD3 aa 1-140 Antibody

Catalog #: AF2819 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2819
AF2819-SP

Detection of Human FoxD3 by Western Blot.

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Human FoxD3 aa 1-140 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FoxD3 (aa 1-140) in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxP3, rhFoxP1, and rhFoxO3A is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FoxD3
Met1-Val140
Accession # Q9UJU5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FoxD3 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human FoxD3. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FoxD3 aa 1-140 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2819) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxD3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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FoxD3 in BG01V Human Stem Cells. FoxD3 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human FoxD3 aa 1-140 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2819) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxD3

FOXD3 (forkhead box protein D3; also HNF3, HFH2 and Genesis) is a 58-60 kDa member of the winged helix transcription factor gene family. It is expressed in the epiblast, migrating neural crest, and neural progenitors of the developing spinal cord. FOXD3 is believed to maintain an uncommitted state in multipotent neural crest stem cells, and to induce cell cycle arrest via p53 and p21^cip1. Human FOXD3 is 478 amino acids (aa) in length. It contains a forkhead DNA binding domain (aa 141‑235) plus an Ala-rich region (aa 262-473). Over aa 1-140 and 302-462, human FOXD3 shares 76% and 91% aa identity with mouse FoxD3, respectively.

Long Name

Forkhead Box D3

Entrez Gene IDs

27022 (Human); 15221 (Mouse)

Alternate Names

AIS1; CWH3; forkhead box D3; forkhead box protein D3; FoxD3; Genesis; HFH2; HNF3/FH transcription factor genesis