Human FLRT3 Biotinylated Antibody

Catalog #: BAF2795 Datasheet / COA / SDS

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BAF2795

All FLRT3 Antibodies

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Human FLRT3 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FLRT3 in Western blots. In Western blots, less than 10% cross-reactivity with recombinant human (rh) FLRT1 and rhFLRT2 is observed.

Source

Polyclonal Goat IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human FLRT3 (Catalog # 2795-FL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: FLRT3

FLRT3 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85-95 kDa type I transmembrane (TM) human FLRT3 is synthesized as a 649 amino acid (aa) precursor with a 28 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa TM segment and a 100 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT3 ECD shares 96%, 96%, 97%, 97%, 98%, and 81% aa sequence identity with mouse, rat, canine, bovine, equine, and Xenopus FLRT3 ECD, respectively, and 61% and 48% aa identity to human FLRT2 and FLRT3 ECDs, respectively. The fibronectin domain is responsible for binding to FGF receptors, and is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization in areas of cell contact and homotypic cell-cell association (4). This may be through direct interaction with other FLRT molecules, or alternatively, by regulating internalization of adhesion molecules such as cadherins (4, 5). Developmentally, FLRT3 is located in somitic regions on dermatomyotomal muscle precursors and myotomal cells before their migration to the myotome and syndetome, respectively (2). FLRT3 is also expressed at the midbrain/hindbrain boundary and in the apical ectodermal ridge where it may influence FGF signaling (2). Genetic deletion in mouse embryos results in defective headfold fusion and endoderm migration (6). Postnatally, FLRT3 mRNA is widely expressed (1). It is up‑regulated and promotes neurite outgrowth following experimental peripheral nerve injury in rats (7, 8).

References

Lacy, S.E. et al. (1999) Genomics 62:417.
Haines, B.P. et al. (2006) Dev. Biol. 297:14.
Bottcher, R.T. et al. (2004) Nat. Cell Biol. 6:38.
Karaulanov, E.E. et al. (2006) EMBO Rep. 7:283.
Ogata, S. et al. (2007) Genes Dev. 21:1817.
Maretto, S. et al. (2008) Dev. Biol. 318:184.
Tsuji, L. et al. (2004) Biochem. Biophys. Res. Commun. 313:1086.
Robinson, M. et al. (2004) Mol. Cell. Neurosci. 27:202.

Long Name

Fibronectin Leucine Rich Transmembrane Protein 3

Entrez Gene IDs

23767 (Human); 71436 (Mouse)

Alternate Names

fibronectin leucine rich transmembrane protein 3; Fibronectin-like domain-containing leucine-rich transmembrane protein 3; FLRT3; HH21; KIAA1469; leucine-rich repeat transmembrane protein FLRT3