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Human FLRT3 Antibody

Catalog #: AF2795
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2795
AF2795-SP
Product Details
Citations (5)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FLRT3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FLRT3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human FLRT1 and rhFLRT2 is observed.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FLRT3 (Catalog # 2795-FL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: FLRT3

FLRT3 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85-95 kDa type I transmembrane (TM) human FLRT3 is synthesized as a 649 amino acid (aa) precursor with a 28 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa TM segment and a 100 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT3 ECD shares 96%, 96%, 97%, 97%, 98%, and 81% aa sequence identity with mouse, rat, canine, bovine, equine, and Xenopus FLRT3 ECD, respectively, and 61% and 48% aa identity to human FLRT2 and FLRT3 ECDs, respectively. The fibronectin domain is responsible for binding to FGF receptors, and is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization in areas of cell contact and homotypic cell-cell association (4). This may be through direct interaction with other FLRT molecules, or alternatively, by regulating internalization of adhesion molecules such as cadherins (4, 5). Developmentally, FLRT3 is located in somitic regions on dermatomyotomal muscle precursors and myotomal cells before their migration to the myotome and syndetome, respectively (2). FLRT3 is also expressed at the midbrain/hindbrain boundary and in the apical ectodermal ridge where it may influence FGF signaling (2). Genetic deletion in mouse embryos results in defective headfold fusion and endoderm migration (6). Postnatally, FLRT3 mRNA is widely expressed (1). It is upregulated and promotes neurite outgrowth following experimental peripheral nerve injury in rats (7, 8).

 

References
  1. Lacy, S.E. et al. (1999) Genomics 62:417.
  2. Haines, B.P. et al. (2006) Dev. Biol. 297:14.
  3. Bottcher, R.T. et al. (2004) Nat. Cell Biol. 6:38.
  4. Karaulanov, E.E. et al. (2006) EMBO Rep. 7:283.
  5. Ogata, S. et al. (2007) Genes Dev. 21:1817.
  6. Maretto, S. et al. (2008) Dev. Biol. 318:184.
  7. Tsuji, L. et al. (2004) Biochem. Biophys. Res. Commun. 313:1086.
  8. Robinson, M. et al. (2004) Mol. Cell. Neurosci. 27:202.
Long Name
Fibronectin Leucine Rich Transmembrane Protein 3
Entrez Gene IDs
23767 (Human); 71436 (Mouse)
Alternate Names
fibronectin leucine rich transmembrane protein 3; Fibronectin-like domain-containing leucine-rich transmembrane protein 3; FLRT3; HH21; KIAA1469; leucine-rich repeat transmembrane protein FLRT3

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Citations for Human FLRT3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Apicosome: Newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cells
    Authors: Xiaofen Li, Qirui Zhao, Xiaojie Yu, Wenhan Cao, Yingyi Zhang, Wanying Feng et al.
    iScience
  2. Multiregional profiling of the brain transmembrane proteome uncovers novel regulators of depression
    Authors: Shanshan Li, Huoqing Luo, Ronghui Lou, Cuiping Tian, Chen Miao, Lisha Xia et al.
    Science Advances
  3. Increased expression of fibronectin leucine-rich transmembrane protein 3 in the dorsal root ganglion induces neuropathic pain in rats
    Authors: M Yamada, Y Fujita, Y Hayano, H Hayakawa, K Baba, H Mochizuki, T Yamashita
    J. Neurosci., 2019-07-25;0(0):.
    Species: Rat
    Sample Types: In Vivo, Tissue Homogenates, Whole Tissue
    Applications: IHC, In Vivo, Western Blot
  4. Genetic ablation of FLRT3 reveals a novel morphogenetic function for the anterior visceral endoderm in suppressing mesoderm differentiation.
    Authors: Egea J, Erlacher C, Montanez E, Burtscher I, Yamagishi S, Hess M, Hampel F, Sanchez R, Rodriguez-Manzaneque MT, Bosl MR, Fassler R, Lickert H, Klein R
    Genes Dev., 2008-12-01;22(23):3349-62.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons
    Authors: Del Toro D, Carrasquero-Ordaz MA, Chu A et al.
    Cell

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