Human Ficolin-2 Antibody

Catalog #: AF2428 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2428
AF2428-SP

All Ficolin-2 Antibodies

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Human Ficolin-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ficolin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human Ficolin-3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Ficolin-2
Leu26-Ala313
Accession # Q15485

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Ficolin‑2 (Catalog # 2428-FC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ficolin-2

Human Ficolin-2 (fibrinogen/collagen-like; previously called L-ficolin or ficolin-B) is a member of the ficolin family of secreted pattern recognition proteins that participate in the lectin complement activation pathway (1‑4). Ficolin-2 is expressed in the liver and released into the circulation (2). The 35‑40 kDa, 313 amino acid (aa) human Ficolin-2 contains a 25 aa signal sequence, an N‑terminal collagen domain and a C‑terminal fibrinogen‑like domain that includes a calcium binding site and two potential N‑glycosylation sites. The collagen domain mediates trimer formation. Larger homo‑multimers are formed by disulfide links at the N‑terminus, the most prominent of which is a 12 subunit oligomer (3, 5). Ficolin‑2 binds microbial ligands that contain acetylated compounds (6). Notably, this includes N‑acetyl glucosamine in compounds such as lipoteichoic acid in gram-positive bacteria. It also binds fungal 1,3-beta -D-glucan (4, 7, 8). Pathogen recognition by Ficolin-2 initiates an immune response that involves calcium-dependent interaction of Ficolin-2 with the MBL-associated serine protease (MASP) complex. This complex cleaves C4 to activate the complement pathway (4, 7, 8). In a secondary role, Ficolin-2 is known to bind late apoptotic and necrotic cells, probably through the recognition of exposed DNA. This also activates the complement cascade that assists in clearance of cells (9, 10). Mature human Ficolin‑2 shares 70%, 72%, 76% and 78% aa identity with mouse, rat, cow and pig Ficolin-2, respectively. It shares 84% and 52% aa identity with human ficolin-1 and ficolin-3, respectively. Single nucleotide polymorphisms are common in human Ficolin‑2. Some affect serum concentration, while others can increase or decrease ligand binding (11).

References

Endo, Y. et al. (2006) Adv. Exp. Med. Biol. 586:265.
Endo, Y. et al. (1996) Genomics 36:515.
Matsushita, M. et al. (1996) J. Biol. Chem. 271:2448.
Ma, Y. G. et al. (2004) J. Biol. Chem. 279:25307.
Hummelshoj, T. et al. (2007) Mol. Immunol. 44:401.
Krarup, A. et al. (2004) J. Biol. Chem. 279:47513.
Garlatti, V. et al. (2007) EMBO J. 26:623.
Lynch, N. J. et al. (2004) J. Immunol. 172:1198.
Jensen, M. L. et al. (2007) Mol. Immunol. 44:856.
Kuraya, M. et al. (2005) Immunobiology 209:689.
Hummelshoj, T. et al. (2005) Hum. Mol. Genet. 14:1651.

Long Name

Ficolin [Collagen/Fibrinogen Domain Containing] 2

Entrez Gene IDs

2220 (Human)

Alternate Names

37 kDa elastin-binding protein; Collagen/fibrinogen domain-containing protein 2; EBP-37ficolin (collagen/fibrinogen domain-containing lectin) 2 (hucolin); FCN2; FCNL; FCNLficolin B; ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin); Ficolin2; Ficolin-2; ficolin-B; ficolin-beta; Hucolin; L-Ficolin; P35; Serum lectin p35