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Human FGFR3 (IIIc) Antibody

Catalog #: MAB7662
3 Citations Datasheet / COA / SDS
Clone 136312 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB7662-SP
MAB7662-500
MAB7662-100
Detection of FGFR3 (IIIc) in HepG2 Human Cell Line by Flow Cytometry.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGFR3 (IIIc) Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF R3 (IIIc) in direct ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) FGF R2 (IIIc), recombinant mouse (rm) FGF R2 (IIIc) and rmFGF R3 (IIIc) is observed. In Western blots (non-reducing conditions only), 50‑100% cross-reactivity with rhFGF R2 (IIIc), rmFGF R2 (IIIc) and rmFGF R3 (IIIc) is observed.
Source
Monoclonal Mouse IgG1 Clone # 136312
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Pool of NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FGF R3 (IIIc) Fc Chimera (Catalog # 766-FR) under non-reducingconditions.
Flow Cytometry
0.25 µg/106 cells
HepG2 human hepatocellular carcinoma cell line
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of FGFR3 (IIIc) in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human FGFR3 (IIIc) Monoclonal Antibody (Catalog # MAB7662, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry View Larger

FGFR3 in HepG2 Human Cell Line. FGFR3 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Mouse Anti-Human FGFR3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry View Larger

FGFR3 in MCF‑7 Human Cell Line. FGFR3 was detected in immersion fixed MCF‑7 human breast cancer cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Mouse Anti-Human FGFR3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry FGF R3 antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

FGF R3 in Human Kidney. FGF R3 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human FGF R3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 8 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR3

FGF R3 is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co-factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF-1, -2, -4, -8b, -8e, -8f, -9 and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Long Name
Fibroblast Growth Factor Receptor 3
Entrez Gene IDs
2261 (Human); 14184 (Mouse)
Alternate Names
CD333; CEK; EC 2.7.10; FGF R3; FGFR3; fibroblast growth factor receptor 3; HSFGFR3EX; JTK4; JTK4thanatophoric dwarfism

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Citations for Human FGFR3 (IIIc) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. In vitro and in vivo characterization of Recifercept, a soluble fibroblast growth factor receptor 3, as treatment for achondroplasia
    Authors: D Gonçalves, G Rignol, P Dellugat, G Hartmann, S Sarrazy Ga, J Stavenhage, L Santarelli, E Gouze, C Czech
    PLoS ONE, 2020-12-28;15(12):e0244368.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Capture
  2. Enhanced Expression of Fibroblast Growth Factor Receptor 3 IIIc Promotes Human Esophageal Carcinoma Cell Proliferation
    Authors: Nobuhiro Ueno, Akio Shimizu, Michiyuki Kanai, Yugo Iwaya, Shugo Ueda, Jun Nakayama et al.
    Journal of Histochemistry & Cytochemistry
  3. Alternative splicing of fibroblast growth factor receptor 3 produces a secreted isoform that inhibits fibroblast growth factor-induced proliferation and is repressed in urothelial carcinoma cell lines.
    Authors: Tomlinson DC, L'Hôte CG, Kennedy W, Pitt E, Knowles MA
    Cancer Res., 2005-11-15;65(22):10441-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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