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Human FGF-9 Antibody

Catalog #: AF-273-NA
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-273-NA
AF-273-SP
FGF‑9 in Human Placenta.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF-9 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FGF‑4, rhFGF-5, rhFGF-6, rhFGF-7, rhFGF-8, rhFGF-10, rhFGF-17, rhFGF-18, and rhFGF-19 is observed.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FGF‑9 (Catalog # 273-F9)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry FGF‑9 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

FGF‑9 in Human Placenta. FGF-9 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Goat Anti-Human FGF-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-273-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: FGF-9

The FGF family is comprised of at least nine polypeptides that show a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. All FGFs have two conserved cysteine residues and share 30-50% sequence identity at the amino acid level. FGF-9, also named glia-activating factor, was originally identified and purified from the supernatant of a human glioma cell line as a heparin-binding mitogenic growth factor for glial cells. FGF-9 has also been shown to stimulate the proliferation of oligodendrocyte type 2 astrocyte progenitor cells, Balb/c3T3 fibroblasts, and PC-12 cells. However, unlike FGF acidic and basic, FGF-9 is not a mitogen for human umbilical vein endothelial cells.

The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a potential N-linked glycosylation site. The native protein is glycosylated. FGF-9 exhibits approximately 30% sequence similarity to other members of the FGF family. Although FGF-9 lacks a typical secretion signal, the protein is secreted efficiently after synthesis. Rat FGF-9 cDNA has been cloned and shown to be highly homologous to human FGF-9. The two proteins differ only in one amino acid residue. The expression of the FGF-9 transcripts has been shown to be restricted to the brain and the kidney.

References
  1. Naruo, K. et al. (1993) J. Biol. Chem. 268:2857.
  2. Miyamoto, M. et al. (1993) Mol. Cell Biol. 13:4251.
Long Name
Fibroblast Growth Factor 9
Entrez Gene IDs
2254 (Human); 14180 (Mouse)
Alternate Names
FGF9; FGF-9; fibroblast growth factor 9 (glia-activating factor); Fibroblast growth factor 9; GAF; glia-activating factor; HBFG-9; HBGF-9; Heparin-binding growth factor 9; MGC119914; MGC119915; SYNS3

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Citations for Human FGF-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Role of Fibroblast Growth Factors in the Crosstalk of Hepatic Stellate Cells and Uveal Melanoma Cells in the Liver Metastatic Niche
    Authors: Tatjana Seitz, Nora John, Judith Sommer, Peter Dietrich, Wolfgang E. Thasler, Arndt Hartmann et al.
    International Journal of Molecular Sciences
  2. Rapid Induction of Lung Adenocarcinoma by Fibroblast Growth Factor 9 Signaling through FGF Receptor 3
    Authors: Yongjun Yin, Tomoko Betsuyaku, Joel R. Garbow, Jinbai Miao, Ramaswamy Govindan, David M. Ornitz
    Cancer Research
  3. Expression of Fibroblast Growth Factor 9 in Normal Human Lung and Idiopathic Pulmonary Fibrosis
    Authors: Emily Coffey, Donna R. Newman, Philip L. Sannes
    Journal of Histochemistry & Cytochemistry
  4. Fibroblast growth factor-9 expression in airway epithelial cells amplifies the type I interferon response and alters influenza A virus pathogenesis
    Authors: Bradley E. Hiller, Yongjun Yin, Yi-Chieh Perng, Ítalo de Araujo Castro, Lindsey E. Fox, Marissa C. Locke et al.
    PLOS Pathogens
  5. Fibroblast Growth Factor 9 is expressed by activated hepatic stellate cells and promotes progression of hepatocellular carcinoma
    Authors: T Seitz, K Freese, P Dietrich, WE Thasler, A Bosserhoff, C Hellerbran
    Sci Rep, 2020-03-11;10(1):4546.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases.
    Authors: Yin H, Frontini M, Arpino J, Nong Z, O'Neil C, Xu Y, Balint B, Ward A, Chakrabarti S, Ellis C, Gros R, Pickering J
    J Biol Chem, 2015-07-16;290(36):22127-42.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Relationship between the localization of fibroblast growth factor 9 in prostate cancer cells and postoperative recurrence.
    Authors: Teishima J, Shoji K, Hayashi T
    Prostate Cancer Prostatic Dis., 2011-10-18;15(1):8-14.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization
  8. Fibroblast growth factor 9 delivery during angiogenesis produces durable, vasoresponsive microvessels wrapped by smooth muscle cells.
    Authors: Frontini MJ, Nong Z, Gros R, Drangova M, O'Neil C, Rahman MN, Akawi O, Yin H, Ellis CG, Pickering JG
    Nat. Biotechnol., 2011-04-17;29(5):421-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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