Home / FGF-16 / Human FGF-16 Antibody

Human FGF-16 Antibody

Catalog #: MAB1212 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1212
MAB1212-SP
Product Details
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-16 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF-16 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) FGF-3, -5, -8c, and -11 is observed and no cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF-4, -6, -7, -8, -9, -10, -12, -13, -17, -18, or -19 is observed.
Source
Monoclonal Mouse IgG2B Clone # 209511
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FGF-16
Ala2-Arg207
Accession # O43320
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FGF‑16 (Catalog # 1212-FG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-16

Fibroblast growth factor 16 (FGF-16) belongs to the large FGF family which has at least 23 members (1). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (2, 3). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the immunoglobulin (Ig) superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (3). Human FGF-16 cDNA predicts a 207 aa precursor protein with one N-linked glycosylation site. FGF-16 lacks a typical signal peptide but is efficiently generated by mechanisms other than the classical protein secretion pathway. Among FGF family members, FGF-16 is most similar to FGF-9, sharing 73% aa sequence homology. Human FGF-16 shares 99% and 98.6% aa sequence identity with the mouse and rat FGF-16, respectively. In rat embryos, FGF-16 message is expressed predominantly in brown adipocytes. In adult animals, it is localized primarily in heart tissue. FGF-16 binds to and activates FGF receptor 4 (4). FGF-16 induces proliferation of primary adipocytes and oligodendrocytes in vitro and stimulates liver weight increase in vivo (4, 5). The expression pattern of FGF-16 and its effect on adipocyte proliferation suggest a role for this protein on the proliferation of embryonic brown adipose tissue (4).

References
  1. Miyake, A. et al. (1998) Biochem. Biophys. Res. Com. 243:148.
  2. Goldfarb, M. (1996) Cytokine and Growth Factor Reviews 7:311.
  3. Green, P. et al. (1996) BioEssays 18:639.
  4. Konishi, M. et al. (2000) J. Biol. Chem. 275:12119.
  5. Danilenko, D.M. et al. (2000) Archiv. Biochem. Biophys. 361:34.
Long Name
Fibroblast Growth Factor 16
Entrez Gene IDs
8823 (Human)
Alternate Names
FGF16; FGF-16; fibroblast growth factor 16

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human FGF-16 Antibody

There are currently no reviews for this product. Be the first to review Human FGF-16 Antibody and earn rewards!

Have you used Human FGF-16 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review