Home / Fc gamma RIIB/C (CD32b/c) / Human Fc gamma  RIIB/C (CD32b/c) Biotinylated Antibody

Human Fc gamma  RIIB/C (CD32b/c) Biotinylated Antibody

Catalog #: BAM1875 Datasheet / COA / SDS
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BAM1875
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Human Fc gamma  RIIB/C (CD32b/c) Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fc gamma RIIB/C in ELISAs. In ELISAs, this antibody shows approximately 40% cross-reactivity with recombinant human (rh) Fc gamma  RIIA and no cross‑reactivity with rhFc gamma RI, rmFc gamma RI, rmFc gamma RIIB, rhFc gamma RIIIB, rmFc gamma RIII, or rmFcrl3/CD16-2.
Source
Monoclonal Mouse IgG2A Clone # 190703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIB/C
Ala46-Pro217
Accession # P31994
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human Fc gamma RIIB/CD32b Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human Fc gamma  RIIB/C (CD32b/c) Antibody (Catalog # MAB18751)

Standard: Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF (Catalog # 1875-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIB/C (CD32b/c)

Fc gamma RII, also known as CD32, is a group of three closely related proteins (Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIC) that share greater than 94% amino acid identity in their extracellular domains. They function as transmembrane receptors for the Fc portion of IgG molecules. These proteins are expressed by various hematopoietic cells including monocytes, macrophages, neutrophils, NK, T and B cells. The Fc gamma RII proteins are involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Long Name
Fc gamma Receptor IIB/C
Entrez Gene IDs
2213 (Human); 14130 (Mouse)
Alternate Names
CD32;CD32B;FCG2;FcgammaRIIb;FCGR2;FCGR2B;FCGR2C;FcGRIIB;FcRII-c;IGFR2;Low affinity immunoglobulin gamma Fc region receptor II-b; Fc gamma RIIB/C (CD32b/c)

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