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Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF

Catalog #: 1875-CD
20 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1875-CD-050
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Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein Binding Activity
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Citations (20)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF Summary

Why choose R&D Systems Fc gamma RIIB/C Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

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Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind human IgG with an estimated Kd <200 nM.
Source
Mouse myeloma cell line, NS0-derived human Fc gamma RIIB/C (CD32b/c) protein
Ala46-Pro217, with a C-terminal 10-His tag
Accession #
P31994
N-terminal Sequence
Analysis
Ala46
Predicted Molecular Mass
21 kDa
SDS-PAGE
25-35 kDa, reducing conditions

Product Datasheets

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1875-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1875-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein Binding Activity View Larger

Recombinant Human Fc gamma RIIB/C (CD32b/c) (Catalog # 1875-CD) binds human IgG with an estimated Kd<200 nM.

SDS-PAGE Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human Fc gamma RIIB/C (CD32b/c) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing multiple bands at 27-35 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIB/C (CD32b/c)

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, FcR gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIB, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three distinct genes encode the human CD32 group, and the protein products are designated Fc gamma  RIIA, B, and C (1). These receptors are glycoproteins of approximately 40 kDa having two extracellular Ig-like domains. The Fc gamma  RII proteins share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fc gamma  RIIA associates with FcR gamma, and delivers an activating signal upon ligand binding (3, 5). In contrast, Fc gamma  RIIB delivers an inhibitory signal. Fc gamma  RIIC represents an unequal cross-over event between the IIA and IIB genes. Its extracellular domain shares 99% amino acid identity with Fc gamma  RIIB, but a portion of the cytoplasmic domain is closely related to Fc gamma  RIIA. Fc gamma  RII proteins are expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin.

References
  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
Long Name
Fc gamma Receptor IIB/C
Entrez Gene IDs
2213 (Human); 14130 (Mouse)
Alternate Names
CD32;CD32B;FCG2;FcgammaRIIb;FCGR2;FCGR2B;FCGR2C;FcGRIIB;FcRII-c;IGFR2;Low affinity immunoglobulin gamma Fc region receptor II-b; Fc gamma RIIB/C (CD32b/c)

Citations for Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Diverging maternal and infant cord antibody functions from SARS-CoV-2 infection and vaccination in pregnancy
    Authors: Adhikari, EH;Lu, P;Kang, YJ;McDonald, AR;Pruszynski, JE;Bates, TA;McBride, SK;Trank-Greene, M;Tafesse, FG;Lu, LL;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. Fc-gamma receptor-mediated crosslinking co-defines the immunostimulatory activity of anti-human CD96 antibodies
    Authors: A Rogel, FM Ibrahim, SM Thirdborou, F Renart-Dep, CN Birts, SL Buchan, X Preville, EV King, A Al-Shamkha
    JCI Insight, 2022-10-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. BNT162b2-induced neutralizing and non-neutralizing antibody functions against SARS-CoV-2 diminish with age
    Authors: TA Bates, P Lu, YJ Kang, D Schoen, M Thornton, SK McBride, C Park, D Kim, WB Messer, ME Curlin, FG Tafesse, LL Lu
    Cell Reports, 2022-10-05;41(4):111544.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Combined IgE neutralization and Bifidobacterium longum supplementation reduces the allergic response in models of food allergy
    Authors: SB An, BG Yang, G Jang, DY Kim, J Kim, SM Oh, N Oh, S Lee, JY Moon, JA Kim, JH Kim, YJ Song, HW Hyun, J Kim, K Lee, D Lee, MJ Kwak, BK Kim, YK Park, CP Hong, JH Kim, HS Lim, MS Ryu, HT Jin, SW Lee, YS Chang, HS Park, YC Sung, MH Jang
    Nature Communications, 2022-09-27;13(1):5669.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  5. BNT162b2 induced neutralizing and non-neutralizing antibody functions against SARSCoV-2 diminish with age
    Authors: TA Bates, P Lu, YJ Kang, D Schoen, M Thornton, SK McBride, C Park, D Kim, WB Messer, ME Curlin, FG Tafesse, LL Lu
    medRxiv : the preprint server for health sciences, 2022-08-16;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  6. Discovery of a novel Igbeta and FcgammaRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus
    Authors: Y Kitanaga, D Yamajuku, S Kubo, K Nakamura, M Maeda, M Seki, Y Kaneko, F Kinugasa, T Morokata, Y Kondo, H Yoshinari, S Nakayamada, T Sumida, Y Tanaka
    International immunopharmacology, 2021-11-12;101(0):108343.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  7. Developing a multivariate prediction model of antibody features associated with protection of malaria-infected pregnant women from placental malaria
    Authors: EH Aitken, T Damelang, A Ortega-Paj, A Alemu, W Hasang, S Dini, HW Unger, M Ome-Kaius, MA Nielsen, A Salanti, J Smith, S Kent, PM Hogarth, BD Wines, JA Simpson, A Chung, SJ Rogerson
    Elife, 2021-06-29;10(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Bioassay
  8. High neutralizing potency of swine glyco-humanized polyclonal antibodies against SARS-CoV-2
    Authors: B Vanhove, O Duvaux, J Rousse, PJ Royer, G Evanno, C Ciron, E Lheriteau, L Vacher, N Gervois, R Oger, Y Jacques, S Conchon, A Salama, R Duchi, I Lagutina, A Perota, P Delahaut, M Ledure, M Paulus, RT So, CK Mok, R Bruzzone, M Bouillet, S Brouard, E Cozzi, C Galli, D Blanchard, JM Bach, JP Soulillou
    European Journal of Immunology, 2021-03-02;0(0):.
    Species: Porcine
    Sample Types: Protein
    Applications: Bioassay
  9. An IgG-based bispecific antibody for improved dual targeting in PSMA-positive cancer
    Authors: L Zekri, F Vogt, L Osburg, S Müller, J Kauer, T Manz, M Pflügler, A Maurer, JS Heitmann, I Hagelstein, M Märklin, S Hörner, T Todenhöfer, C Calaminus, A Stenzl, B Pichler, C la Fougère, MA Schneider, HG Rammensee, L Zender, B Sipos, HR Salih, G Jung
    Embo Molecular Medicine, 2020-12-29;0(0):e11902.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  10. Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus
    Authors: PA Blundell, D Lu, M Wilkinson, A Dell, S Haslam, RJ Pleass
    J. Immunol., 2019-01-25;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)
  11. Analytical and functional similarity of Amgen biosimilar ABP 215 to bevacizumab
    Authors: N Seo, A Polozova, M Zhang, Z Yates, S Cao, H Li, S Kuhns, G Maher, HJ McBride, J Liu
    MAbs, 2018-04-20;0(0):1-58.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  12. Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies
    Authors: F Arce Varga, AJS Furness, K Litchfield, K Joshi, R Rosenthal, E Ghorani, I Solomon, MH Lesko, N Ruef, C Roddie, JY Henry, L Spain, A Ben Aissa, A Georgiou, YNS Wong, M Smith, D Strauss, A Hayes, D Nicol, T O'Brien, L Mårtensson, A Ljungars, I Teige, B Frendéus, M Pule, T Marafioti, M Gore, J Larkin, S Turajlic, C Swanton, KS Peggs, SA Quezada
    Cancer Cell, 2018-03-22;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  13. Fc?RII-binding Centyrins mediate agonism and antibody-dependent cellular phagocytosis when fused to an anti-OX40 antibody
    Authors: D Zhang, B Whitaker, MG Derebe, ML Chiu
    MAbs, 2018-01-29;0(0):1-13.
    Species: Human
    Sample Types: Recombinant Protein
  14. Enzymatic inactivation of endogenous IgG by IdeS enhances�therapeutic antibody efficacy
    Authors: S Järnum, A Runström, R Bockermann, L Winstedt, M Crispin, C Kjellman
    Mol. Cancer Ther., 2017-05-22;0(0):.
    Species: Human
    Sample Types: Antibody
    Applications: Bioassay
  15. GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins.
    Authors: Meuris L, Santens F, Elson G, Festjens N, Boone M, Dos Santos A, Devos S, Rousseau F, Plets E, Houthuys E, Malinge P, Magistrelli G, Cons L, Chatel L, Devreese B, Callewaert N
    Nat Biotechnol, 2014-04-20;32(5):485-9.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  16. Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases.
    Authors: Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex-Rabe S, Yang X, Zhou X, Zhang Y, Gillooly K, Brosius R, Ravishankar R, Waggie K, Mink K, Price L, Rehfuss R, Tamura J, An Y, Cheng L, Abramczyk B, Ignatovich O, Drew P, Grant S, Bryson J, Suchard S, Salter-Cid L, Nadler S, Suri A
    J Immunol, 2014-03-26;192(9):4083-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  17. Increasing FcgammaRIIa affinity of an FcgammaRIII-optimized anti-EGFR antibody restores neutrophil-mediated cytotoxicity.
    Authors: Derer S, Glorius P, Schlaeth M, Lohse S, Klausz K, Muchhal U, Desjarlais J, Humpe A, Valerius T, Peipp M
    MAbs, 2013-12-11;6(2):409-21.
    Applications: Surface Plasmon Resonance
  18. Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors.
    Authors: Franco A, Damdinsuren B, Ise T, Dement-Brown J, Li H, Nagata S, Tolnay M
    J Immunol, 2013-04-24;190(11):5739-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  19. Development and characterisation of monoclonal antibodies specific for the murine inhibitory FcgammaRIIB (CD32B).
    Eur J Immunol, 2012-07-04;42(8):2109-20.
    Applications: Surface Plasmon Resonance
  20. Optimization of antibody binding to FcgammaRIIa enhances macrophage phagocytosis of tumor cells.
    Authors: Richards JO, Karki S, Lazar GA, Chen H, Dang W, Desjarlais JR
    Mol. Cancer Ther., 2008-08-01;7(8):2517-27.
    Species: Human
    Sample Types: Antibody
    Applications: Surface Plasmon Resonance

FAQs

  1. Why does the Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF (Catalog # 1875-CD) state "B/C" in the product name?

    • Catalog # 1875-CD is named Recombinant Human Fc gamma RIIB/C because the sequence of our protein (Ala46-Pro217) corresponds to both the B and the C forms. Fc gamma RII B and C only differ in the C-terminal portion of the sequence not included in this protein product.

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