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Human FATP1 Antibody

Catalog #: MAB3304
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3304
MAB3304-SP
FATP1 in HeLa Human Cell Line.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FATP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FATP1 in cell transfectants but not non-transfected parent cells in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 308420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human FATP1
Met1-Leu646
Accession # Q6PCB7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
HT‑29 human colon adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry FATP1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

FATP1 in HeLa Human Cell Line. FATP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human FATP1 Monoclonal Antibody (Catalog # MAB3304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FATP1

FATP1 is a plasma membrane protein with 6 transmembrane domains. It is involved in translocation of long-chain fatty acids across the plasma membrane. FATP1 is expressed in muscle, intestine, and adipose tissue.

Long Name
Fatty Acid Transport Protein 1
Entrez Gene IDs
376497 (Human); 26457 (Mouse); 94172 (Rat)
Alternate Names
ACSVL5FATP-1; EC 6.2.1; EC 6.2.1.-; FATP1; FATP1Solute carrier family 27 member 1; FATPlong-chain fatty acid transport protein 1; Fatty acid transport protein 1; FLJ00336; MGC71751; SLC27A1; solute carrier family 27 (fatty acid transporter), member 1

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Citations for Human FATP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The Phenotype of the Adipocytes Derived from Subcutaneous and Visceral ADMSCs Is Altered When They Originate from Morbidly Obese Women: Is There a Memory Effect?
    Authors: A Mik?osz, B ?ukaszuk, E Supruniuk, K Grubczak, A Starosz, M Kusaczuk, M Naumowicz, A Chabowski
    Cells, 2022-04-23;11(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment
    Authors: M Bou, JS Torgersen, TK Østbye, B Ruyter, X Wang, S Škugor, IØ Kristianse, M Todor?evi?
    Int J Mol Sci, 2020-06-08;21(11):.
    Species: Fish - Atlantic Salmon
    Sample Types: Whole Cells
    Applications: ICC
  3. Unraveling FATP1, regulated by ER-beta, as a targeted breast cancer innovative therapy
    Authors: C Mendes, F Lopes-Coel, C Ramos, F Martins, I Santos, A Rodrigues, F Silva, S André, J Serpa
    Sci Rep, 2019-10-01;9(1):14107.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  4. The blood-brain barrier fatty acid transport protein 1 (FATP1/SLC27A1) supplies docosahexaenoic acid to the brain, and insulin facilitates transport
    Authors: Yusuke Ochiai
    J. Neurochem, 2017-03-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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