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Human FABP7/B-FABP Antibody

Catalog #: AF3166
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3166
AF3166-SP
FABP7 in Human Melanoma.
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Product Details
Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FABP7/B-FABP Antibody Summary

Species Reactivity
Human
Specificity
Detects human FABP7/B-FABP in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP6, rhFABP8, rhFABP9 and recombinant mouse FABP9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FABP7/B-FABP
Met1-Ala132
Accession # O15540
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FABP7
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry FABP7 antibody in Human Melanoma by Immunohistochemistry (IHC-P). View Larger

FABP7 in Human Melanoma. FABP7 was detected in immersion fixed paraffin-embedded sections of human melanoma using 5 µg/mL Goat Anti-Human FABP7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human FABP7/B-FABP antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human FABP7/B‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human cerebellum tissue and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP7/B-FABP at approximately 18 kDa (as indicated) using 50 µg/mL of Goat Anti-Human FABP7/B-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP7/B-FABP

FABP7, also known as (Brain) B-FABP, Brain lipid-binding protein (BLBP), and mammary-derived growth inhibitor-related (MRG), belongs to the family of intracellular lipid-binding proteins. It is involved in fatty acid uptake and intracellular transport and is important in brain development. Human and mouse FABP7 share 86% amino acid sequence identity.

Long Name
Fatty Acid-Binding Protein 7
Entrez Gene IDs
2173 (Human); 12140 (Mouse); 80841 (Rat)
Alternate Names
BFABP; B-FABP; B-FABPDKFZp547J2313; BLBP; BLBPMRG; Brain lipid-binding protein; Brain-type fatty acid-binding protein; FABP7; FABPBbrain lipid binding protein; fatty acid binding protein 7, brain; Fatty acid-binding protein 7; fatty acid-binding protein, brain; Mammary-derived growth inhibitor related; mammary-derived growth inhibitor-related

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Citations for Human FABP7/B-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Discrepancy in the Usage of GFAP as a Marker of Satellite Glial Cell Reactivity
    Authors: Kjeld Morten Mohr, Lone Tjener Pallesen, Mette Richner, Christian Bjerggaard Vaegter
    Biomedicines
  2. Tiered sympathetic control of cardiac function revealed by viral tracing and single cell transcriptome profiling
    Authors: Sharma S, Littman R, Tompkins JD et al.
    eLife
  3. Tiered sympathetic control of cardiac function revealed by viral tracing and single cell transcriptome profiling
    Authors: Sharma S, Littman R, Tompkins JD et al.
    eLife
  4. Tiered Sympathetic Control of Cardiac Function Revealed by Viral Tracing and Single Cell Transcriptome Profiling
    Authors: S Sharma, R Littman, J Tompkins, D Arneson, J Contreras, AH Dajani, K Ang, A Tsanhani, X Sun, PY Jay, H Herzog, X Yang, OA Ajijola
    bioRxiv : the preprint server for biology, 2023-01-19;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. DRGquant: A new modular AI-based pipeline for 3D analysis of the DRG
    Authors: MA Hunt, H Lund, L Delay, GG Dos Santos, A Pham, Z Kurtovic, A Telang, A Lee, A Parvathane, E Kussick, M Corr, TL Yaksh
    Journal of neuroscience methods, 2022-02-16;371(0):109497.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. A novel fatty acid-binding protein 5 and 7 inhibitor ameliorates oligodendrocyte injury in multiple sclerosis mouse models
    Authors: A Cheng, W Jia, I Kawahata, K Fukunaga
    EBioMedicine, 2021-10-05;72(0):103582.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  7. Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice
    Authors: Q Guo, I Kawahata, T Degawa, Y Ikeda-Mats, M Sun, F Han, K Fukunaga
    Biomedicines, 2021-05-10;9(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Fatty Acid Binding Protein 5 Mediates Cell Death by Psychosine Exposure through Mitochondrial Macropores Formation in Oligodendrocytes
    Authors: A Cheng, I Kawahata, K Fukunaga
    Biomedicines, 2020-12-20;8(12):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation, Western Blot
  9. Imbalance of Excitatory/Inhibitory Neuron Differentiation in Neurodevelopmental Disorders with an NR2F1 Point Mutation
    Authors: K Zhang, F Yu, J Zhu, S Han, J Chen, X Wu, Y Chen, T Shen, J Liao, W Guo, X Yang, R Wang, Y Qian, J Yang, L Cheng, Y Zhao, CC Hui, J Li, G Peng, S He, N Jing, K Tang
    Cell Rep, 2020-04-21;31(3):107521.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Meis1 coordinates cerebellar granule cell development by regulating Pax6 transcription, BMP signaling and Atoh1 degradation
    Authors: T Owa, S Taya, S Miyashita, M Yamashita, T Adachi, K Yamada, M Yokoyama, S Aida, T Nishioka, YU Inoue, R Goitsuka, T Nakamura, T Inoue, K Kaibuchi, M Hoshino
    J. Neurosci., 2018-01-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Functional analysis of fatty acid binding protein 7 and its effect on fatty acid of renal cell carcinoma cell lines
    Authors: N Takaoka, T Takayama, S Ozono
    BMC Cancer, 2017-03-14;17(1):192.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Expression of fatty acid-binding proteins in adult hippocampal neurogenic niche of postischemic monkeys.
    Authors: Boneva NB, Kaplamadzhiev DB, Sahara S, Kikuchi H, Pyko IV, Kikuchi M, Tonchev AB, Yamashima T
    Hippocampus, 2011-02-01;21(2):162-71.
    Species: Primate - Macaca fuscata (Japanese Macaque)
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  13. Infiltrative and drug‐resistant slow‐cycling cells support metabolic heterogeneity in glioblastoma
    Authors: Lan B Hoang‐Minh, Florian A Siebzehnrubl, Changlin Yang, Silveli Suzuki‐Hatano, Kyle Dajac, Tyler Loche et al.
    The EMBO Journal
  14. Fatty Acid Binding Protein 7 Is a Molecular Marker in Adenoid Cystic Carcinoma of the Salivary Glands: Implications for Clinical Significance12
    Authors: Janyaporn Phuchareon, Jonathan B. Overdevest, Frank McCormick, David W. Eisele, Annemieke van Zante, Osamu Tetsu
    Translational Oncology
  15. Analysis of the regulation of fatty acid binding protein 7 expression in human renal carcinoma cell lines
    Authors: Naohisa Takaoka, Tatsuya Takayama, Takumi Teratani, Takayuki Sugiyama, Soichi Mugiya, Seiichiro Ozono
    BMC Molecular Biology

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