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Human EPCR Antibody

Catalog #: AF2245
17 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2245
AF2245-SP
Detection of Human EPCR by Western Blot.
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Product Details
Citations (17)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EPCR Antibody Summary

Species Reactivity
Human
Specificity
Detects human EPCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse EPCR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EPCR
Ser18-Ser210
Accession # Q9UNN8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
HUVEC human umbilical vein endothelial cells
Immunohistochemistry
1-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EPCR antibody by Western Blot. View Larger

Detection of Human EPCR by Western Blot. Western blot shows lysates of human skin tissue, human placenta tissue, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EPCR at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry EPCR antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

EPCR in Human Liver. EPCR was detected in immersion fixed paraffin-embedded sections of human liver using 1.7 µg/mL Goat Anti-Human EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2245) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EPCR

EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes.  The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology.

Long Name
Endothelial Protein C Receptor
Entrez Gene IDs
10544 (Human); 19124 (Mouse)
Alternate Names
APC receptor; CCCA; CCD41; CCD41centrosome-associated protein; CD201 antigen; CD201; centrocyclin; Endothelial cell protein C receptor; endothelial protein C receptor; EPCR; EPCRMGC23024; PROCR; protein C receptor, endothelial

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Citations for Human EPCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Human platelets express endothelial protein C receptor, which can be utilized to enhance localization of factor VIIa activity
    Authors: A. M. Fager, K. R. Machlus, M. Ezban, M. Hoffman
    Journal of Thrombosis and Haemostasis
  2. Endothelial protein C receptor expressed by ovarian cancer cells as a possible biomarker of cancer onset
    Authors: ELODIE DUCROS, SHAHSOLTAN MIRSHAHI, DALEL AZZAZENE, SOPHIE CAMILLERI-BROËT, ELIANE MERY, HALEMA AL Al Farsi et al.
    International Journal of Oncology
  3. An optimized isolation of biotinylated cell surface proteins reveals novel players in cancer metastasis.
    Authors: Karhemo PR, Ravela S, Laakso M et al.
    J Proteomics.
  4. Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients
    Authors: L Di Pietro, F Giacalone, E Ragozzino, V Saccone, F Tiberio, M De Bardi, M Picozza, G Borsellino, W Lattanzi, E Guadagni, S Bortolani, G Tasca, E Ricci, O Parolini
    Cell Death & Disease, 2022-09-16;13(9):793.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. EPCR-PAR1 biased signaling regulates perfusion recovery and neovascularization in peripheral ischemia
    Authors: ML Bochenek, R Gogiraju, S Gro beta mann, J Krug, J Orth, S Reyda, GS Georgiadis, H Spronk, S Konstantin, T Münzel, JH Griffin, PS Wild, C Espinola-K, W Ruf, K Schäfer
    JCI Insight, 2022-07-22;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  6. Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption
    Authors: M Avril, M Benjamin, MM Dols, JD Smith
    Sci Rep, 2019-09-11;9(1):13142.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes
    Authors: G Ecklu-Mens, RW Olsen, A Bengtsson, MF Ofori, L Hviid, ATR Jensen, Y Adams
    PLoS ONE, 2018-10-09;13(10):e0204177.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  8. An ADAM-10 dependent EPCR shedding links meningococcal interaction with endothelial cells to purpura fulminans
    Authors: H Lécuyer, Z Virion, JP Barnier, S Matczak, S Bourdoulou, E Bianchini, F Saller, D Borgel, X Nassif, M Coureuil
    PLoS Pathog., 2018-04-09;14(4):e1006981.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  9. Infected erythrocytes expressing DC13 PfEMP1 differ from recombinant proteins in EPCR-binding function
    Authors: Y Azasi, G Lindergard, A Ghumra, J Mu, LH Miller, JA Rowe
    Proc. Natl. Acad. Sci. U.S.A., 2018-01-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  10. Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial Cells
    MBio, 2016-07-12;7(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Cellular regulation of blood coagulation: a model for venous stasis.
    Authors: Campbell JE, Brummel-Ziedins KE, Butenas S
    Blood, 2010-09-23;116(26):6082-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
    Authors: Gunawardana CG, Kuk C, Smith CR
    J. Proteome Res., 2009-10-01;8(10):4705-13.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. Aldosterone modifies hemostasis via upregulation of the protein-C receptor in human vascular endothelium.
    Authors: Ducros E, Berthaut A, Mirshahi SS, Faussat AM, Soria J, Agarwal MK, Mirshahi M
    Biochem. Biophys. Res. Commun., 2008-06-13;373(2):192-6.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Dot Blot, Flow Cytometry, ICC
  14. Protein C is an autocrine growth factor for human skin keratinocytes.
    Authors: Xue M, Campbell D, Jackson CJ
    J. Biol. Chem., 2007-02-09;282(18):13610-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Plasma endothelial protein C receptor influences innate immune response in ovarian cancer by decreasing the population of natural killer and TH17 helper cells
    Authors: DALEL AZZAZENE, HAMDA AL Al Thawadi, HALEMA AL Al Farsi, SAMAHER BESBES, CAROLINE GEYL, SHAHSOLTAN MIRSHAHI et al.
    International Journal of Oncology
  16. Soluble endothelial protein C receptor (sEPCR) is likely a biomarker of cancer‐associated hypercoagulability in human hematologic malignancies
    Authors: Elodie Ducros, Shah Soltan Mirshahi, Anne‐Marie Faussat, Pezhman Mirshahi, Sophie Dimicoli, Ruoping Tang et al.
    Cancer Medicine
  17. Role of thrombomodulin expression on hematopoietic stem cells
    Authors: Sreemanti Basu, Hai Po Helena Liang, Irene Hernandez, Mark Zogg, British Fields, Jennifer May et al.
    Journal of Thrombosis and Haemostasis

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