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Human Endothelin-1 C-Terminus Antibody

Catalog #: MAB3440
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3440
MAB3440-SP
Endothelin‑1 in HUVEC Human Cells.
3 Images
Product Details
Citations (2)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Endothelin-1 C-Terminus Antibody Summary

Species Reactivity
Human
Specificity
Detects human Endothelin‑1 C-Terminus. Recognizes the C-terminal region of mature Endothelin-1, -2, and -3 (amino acid residues 15‑21).
Source
Monoclonal Rat IgG2B Clone # 3G10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Endothelin-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Endothelin‑1 C-Terminus Monoclonal Antibody (Catalog # MAB34401).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Endothelin Pan Specific DuoSet ELISA Kit (Catalog # DY1160) for convenient development of a sandwich ELISA or the Endothelin-1 Quantikine ELISA Kit (Catalog # DET100) for a complete optimized ELISA.

 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Endothelin-1 antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

Endothelin‑1 in HUVEC Human Cells. Endothelin-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ELISA Human Endothelin-1 Antibody in ELISA Standard Curve. View Larger

Human Endothelin‑1 ELISA Standard Curve. Recombinant Human Endothelin-1 protein was serially diluted 2-fold and captured by Rat Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB34401) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Immunohistochemistry Detection of Human Endothelin-1 by Immunohistochemistry View Larger

Detection of Human Endothelin-1 by Immunohistochemistry Double staining for CD31 and ET-1 in skin tissue fromthe amputated leg of CRPS patient B. CD31-positive blood vessels are stainedred and ET-1-positive cells are stained blue. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/18663383), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endothelin-1

Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield inactive big endothelins. Big Endothelins are further cleaved to generate the 21 amino acid bioactive mature peptides. Endothelin-1 has potent vasoconstricting and angiogenic activity that is mediated through the 7TM receptors, ET-A and ET-B. Endothelin-1, -2, and -3 share identical amino acid sequences at their C-termini (residues 15‑21).

Entrez Gene IDs
1906 (Human); 13614 (Mouse); 24323 (Rat)
Alternate Names
ARCND3; EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME

Product Datasheets

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Citations for Human Endothelin-1 C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Increased mortality with elevated plasma endothelin-1 in acute heart failure: an ASCEND-HF biomarker substudy.
    Authors: Perez A, Grodin J, Wu Y, Hernandez A, Butler J, Metra M, Felker G, Voors A, McMurray J, Armstrong P, Starling R, O'Connor C, Tang W
    Eur J Heart Fail, 2015-12-14;18(3):290-7.
    Species: Human
    Sample Types: Plasma
  2. Expression of endothelial nitric oxide synthase and endothelin-1 in skin tissue from amputated limbs of patients with complex regional pain syndrome.
    Authors: Groeneweg JG, Antonissen CH, Huygen FJ
    Mediators Inflamm., 2008-01-01;2008(0):680981.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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