Human Endoglin/CD105 Antibody Summary
Glu26-Gly586
Accession # Q5T9B9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human Endoglin/CD105 antibody by Western Blot. Detection of Human Endoglin/CD105 antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/Endoglin_AF1097_Western_Blot_17307.jpg)
Detection of Human Endoglin/CD105 by Western Blot. Western blot shows lysates of human kidney tissue, human lung tissue, human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Endoglin/CD105 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![Endoglin/CD105 antibody in U937 Human Cell Line by Immunocytochemistry (ICC). Endoglin/CD105 antibody in U937 Human Cell Line by Immunocytochemistry (ICC).](https://resources.rndsystems.com/images/datasheets/antibody/Endoglin_AF1097_Immunocytochemistry_7255.jpg)
Endoglin/CD105 in U937 Human Cell Line. Endoglin/CD105 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
![Endoglin/CD105 antibody in Human Prostate by Immunohistochemistry (IHC-P). Endoglin/CD105 antibody in Human Prostate by Immunohistochemistry (IHC-P).](https://resources.rndsystems.com/images/datasheets/antibody/Endoglin_AF1097_Immunohistochemistry_7106.jpg)
Endoglin/CD105 in Human Prostate. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
![Endoglin/CD105 antibody in Mouse Kidney by Immunohistochemistry (IHC-P). Endoglin/CD105 antibody in Mouse Kidney by Immunohistochemistry (IHC-P).](https://resources.rndsystems.com/images/datasheets/antibody/Endoglin_AF1097_Immunohistochemistry_16085.jpg)
Endoglin/CD105 in Mouse Kidney. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
![Detection of Human Endoglin/CD105 antibody by Simple Western<sup>TM</sup>. Detection of Human Endoglin/CD105 antibody by Simple Western<sup>TM</sup>.](https://resources.rndsystems.com/images/datasheets/antibody/Endoglin_AF1097_Simple_Western_22636.jpg)
Detection of Human Endoglin/CD105 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Endoglin/CD105 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Endoglin/CD105 at approximately 121 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) was used at 20 µg/mL followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
![Western Blot Shows Human Endoglin/CD105 Antibody Specificity by Using Knockout Cell Line. Western Blot Shows Human Endoglin/CD105 Antibody Specificity by Using Knockout Cell Line.](https://resources.rndsystems.com/images/datasheets/antibody/Endoglin_AF1097_Knockout_Validated_22587.jpg)
Western Blot Shows Human Endoglin/CD105 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Endoglin/CD105 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Endoglin/CD105 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![Detection of Human Endoglin/CD105 by Immunocytochemistry/Immunofluorescence Detection of Human Endoglin/CD105 by Immunocytochemistry/Immunofluorescence](https://resources.rndsystems.com/images/datasheets/af1097_human-endoglin-cd105-affinity-purified-polyclonal-ab-4120241032146.jpg)
Detection of Human Endoglin/CD105 by Immunocytochemistry/Immunofluorescence Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to repeated cycles of staining and imaging with fluorescent primary antibodies and DAPI. At the end of each cycle, fluorescent signal was removed by a chemical bleaching process, and slides were again imaged, before proceeding to the next round of this iterative procedure. After the final antibody stain (#15 Sma), slides were analyzed with a series of histochemical stains. (b) A set of tiling images spanning each tissue section was initially generated by the microscope system. The tiling images were then computationally ‘stitched’ together to produce a single image per staining cycle for each sample. (c) Image registration was performed to align images from the same tissue section across cycles. Mean intensities of the DAPI signal from all immuno-fluorescence images are shown from before (Unregistered) and after (Registered) the image registration procedure was completed. (d) Following registration, signal intensities from the relevant channels for each image (columns) in the MMMP series were extracted for each pixel (rows) within the tissue section and compiled into a large data matrix of in situ molecular profiles. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0128975), licensed under a CC-BY license. Not internally tested by R&D Systems.
![Detection of Human Endoglin/CD105 by Flow Cytometry Detection of Human Endoglin/CD105 by Flow Cytometry](https://resources.rndsystems.com/images/datasheets/af1097_human-endoglin-cd105-affinity-purified-polyclonal-ab-41202410485447.jpg)
Detection of Human Endoglin/CD105 by Flow Cytometry Transfection of stem cells with mRNA nanoparticles can promote their expansion and self-renewal. a Targeting of CD105 enables specific transfection of HSC CD34+ cells. Cells were left untreated, or transfected with eGFP-encoding mRNA in nanoparticles (NPs) coated with PGA coupled to a control antibody or anti-CD105. Transfection efficiency was assayed by flow cytometry after 24 h. b NP transfection efficiency in CD34+ samples from three independent donors. Viability is shown in c. d Expansion of CD34 + PBSCs after NP-transfection. e Phenotypical characterization of PBSC-derived CD34+ subpopulations after 2 days in culture. Cells were either transfected with eGFP NPs on day 1 or left unmodified. Gating is indicated in brackets on top of each column. f Summary bar graph showing mean frequencies and SE of primitive Hematopoietic Stem Cells (HSCs), Multipotent Progenitors (MPPs), Lymphoid-primed Multipotent Progenitors (LMPs), and Early Myeloid Progenitors (EMPs). PBSCs from four independent donors were analyzed. Error bars represent mean ± SE. g Colony output of sort-purified GFP-NP transfected versus unmodified CD34+ cells from day 7 cultures (n = 3 cultures from independent donors); n.s., non-significant. Arising colonies were identified as colony forming unit (CFU) granulocyte (CFU-G), macrophage (CFU-M), granulocyte-macrophage (CFU-GM) and burst forming unit-erythrocyte (BFU-E). Colonies consisting of erythroid and myeloid cells were scored as CFU-MIX; n.s., non-significant. Error bars represent mean ± SE. h Representative images of CFU-MIX colonies from untransfected and GFP-NP-transfected CD34+ cells (×4-magnification; scale bar 1000 µm). i qPCR measurements of NP-delivered Musashi-2 (MSI2) mRNA expression over time. Error bars represent mean ± SE. j Comparison of CD133 and CD34 expression in HSCs transfected with control GFP mRNA-NPs versus MSI2 mRNA-NPs, assessed by flow cytometry 8 days after NP exposure and cell expansion. Data represent two independent experiments conducted in triplicate. k Cellular fold expansion of CD34− (differentiated) and CD34+ CD133+ (progenitor) cells. Bar graphs show mean and SE of three independent experiments. Data represent two independent experiments conducted in triplicate. l Colony forming unit outputs of untransfected versus MSI2-NP-transfected HSCs (n = 3 cultures from independent donors); Pairwise differences between groups were analyzed with the unpaired, two-tailed Student’s t Test. *P = 0.049, **P = 0.012, ***P = 0.011; n.s., non-significant. Error bars represent mean ± SE. m Representative images of colonies from untransfected and MSI2-NP-transfected CD34+ cells (scale bar, 300 µm) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28855514), licensed under a CC-BY license. Not internally tested by R&D Systems.
![Detection of Human Endoglin/CD105 by Immunocytochemistry/Immunofluorescence Detection of Human Endoglin/CD105 by Immunocytochemistry/Immunofluorescence](https://resources.rndsystems.com/images/datasheets/af1097_human-endoglin-cd105-affinity-purified-polyclonal-ab-4120241048118.jpg)
Detection of Human Endoglin/CD105 by Immunocytochemistry/Immunofluorescence Spatiotemporal changes in tumor vascularization and COX2 coverage at different stages of prostate cancer progression/regression. The 5-μm thick paraffin serial sections were stained with antibodies against endoglin (CD105, red), cyclooxygenase 2 (COX2, green), and CD68 (white). (A–D) Representative 200× pictures are showing changes in vascularization and COX2 expression in tertiary lymphoid organs (TLO), or (E–H) tumor areas. (A) CD105+ vessels, CD68+COX2+ macrophages, and CD68−COX2+ cells are detected in TLO from prostatic intraepithelial neoplasia (PIN) patients. (B) Smaller CD105+ vessels and CD68−COX2+ cells are located on the border of TLO from intermediate prostate cancer patient. (C) In a prostatectomy specimen from advanced carcinoma patient, CD105+ vessels with cuboidal morphology are located outside TLO, while numerous CD68−COX2+ cells are found in the center of a TLO. (D) Oval-shaped and spindle-shaped COX2+ cells are located on the border of a TLO. (E) Vascularity and COX2+ epithelial cells are modestly increased in tumor areas from PIN patients. (F,G) Abundant blood vessels with abnormal morphology and aberrant organization were located in close proximity to strongly labeled COX2+ transformed epithelium in tumors of intermediate and advanced prostate cancer patients. (D,H) Preserved vascular morphology and organization, as well as reduced COX2+ density were observed in TLO and tumor areas from patients with spontaneous prostate cancer regression. CD105+ vessels with a high endothelial venule-like morphology were detected in TLO from patients at late stages of prostate cancer (white asterisk). Representative 200× magnification pictures of TLO and tumor areas were taken with a Zeiss Axioplan Microscope and recorded with a Hamamatsu Camera. Scale bars represent 100 μm. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fimmu.2017.00563/full), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endoglin/CD105
Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and Betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human endoglin cDNA encodes 658 amino acids including a 25 amino acid (aa) signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) endoglin (6, 7). The human endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine, and canine endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse endoglin causes lethal vascular and cardiovascular defects, and human endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (sFlt-1), along with low Placental Growth Factor (PlGF), are pathogenic due to antiangiogenic activity (15).
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Product Datasheets
Citations for Human Endoglin/CD105 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Defective fluid shear stress mechanotransduction mediates hereditary hemorrhagic telangiectasia
Authors: Nicolas Baeyens, Bruno Larrivée, Roxana Ola, Brielle Hayward-Piatkowskyi, Alexandre Dubrac, Billy Huang et al.
Journal of Cell Biology
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CD105 expression in cancer-associated fibroblasts: a biomarker for bone metastasis in early invasive ductal breast cancer patients
Authors: María Belén Giorello, Leandro Marcelo Martinez, Francisco Raúl Borzone, María Del Rosario Padin, María Florencia Mora, Ina Sevic et al.
Front Cell Dev Biol
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Endoglin Is an Important Mediator in the Final Common Pathway of Chronic Kidney Disease to End-Stage Renal Disease
Authors: T Gerrits, IJ Brouwer, KL Dijkstra, R Wolterbeek, JA Bruijn, M Scharpfene, HJ Baelde
International Journal of Molecular Sciences, 2022-12-30;24(1):.
Species: Human
Sample Types: Protein Lysates, Whole Tissue
Applications: IHC, Western Blot -
Atheroprone fluid shear stress-regulated ALK1-Endoglin-SMAD signaling originates from early endosomes
Authors: PL Mendez, L Obendorf, J Jatzlau, W Burdzinski, M Reichenbac, V Nageswaran, A Haghikia, V Stangl, C Hiepen, P Knaus
Bmc Biology, 2022-09-28;20(1):210.
Species: Human
Sample Types: Cell Lysates, Transfected Whole Cells
Applications: IHC, Western Blot -
Single-nucleus transcriptome analysis reveals disease- and regeneration-associated endothelial cells in white matter vascular dementia
Authors: DN Mitroi, M Tian, R Kawaguchi, WE Lowry, ST Carmichael
Journal of Cellular and Molecular Medicine, 2022-05-11;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Reversing pathology in a preclinical model of Alzheimer's disease by hacking cerebrovascular neoangiogenesis with advanced cancer therapeutics
Authors: CSB Singh, KB Choi, L Munro, HY Wang, CG Pfeifer, WA Jefferies
EBioMedicine, 2021-09-15;71(0):103503.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Thresholds of Endoglin Expression in Endothelial Cells Explains Vascular Etiology in Hereditary Hemorrhagic Telangiectasia Type 1
Authors: G Galaris, K Montagne, JH Thalgott, GJPE Goujon, S van den Dr, S Martin, HJ Mager, CL Mummery, TJ Rabelink, F Lebrin
International Journal of Molecular Sciences, 2021-08-19;22(16):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Selection of Highly Proliferative and Multipotent Meniscus Progenitors through Differential Adhesion to Fibronectin: A Novel Approach in Meniscus Tissue Engineering
Authors: JV Korpershoe, M Rikkers, TS de Windt, MA Tryfonidou, DBF Saris, LA Vonk
International Journal of Molecular Sciences, 2021-08-10;22(16):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Endothelium-derived stromal cells contribute to hematopoietic bone marrow niche formation
Authors: KJG Kenswil, P Pisterzi, G Sánchez-Du, C van Dijk, A Lolli, C Knuth, B Vanchin, AC Jaramillo, RM Hoogenboez, MA Sanders, J Feyen, T Cupedo, IG Costa, R Li, EMJ Bindels, K Lodder, B Blom, PK Bos, MJ Goumans, P Ten Dijke, E Farrell, G Krenning, MHGP Raaijmaker
Cell Stem Cell, 2021-02-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Endoglin Promotes Myofibroblast Differentiation and Extracellular Matrix Production in Diabetic Nephropathy
Authors: T Gerrits, M Zandbergen, R Wolterbeek, JA Bruijn, HJ Baelde, M Scharpfene
Int J Mol Sci, 2020-10-18;21(20):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
CD90 determined two subpopulations of glioma-associated mesenchymal stem cells with different roles in tumour progression
Authors: Q Zhang, DY Yi, BZ Xue, WW Wen, YP Lu, A Abdelmakso, MX Sun, DT Yuan, HY Zhao, NX Xiong, W Xiang, P Fu
Cell Death Dis, 2018-10-27;9(11):1101.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Hit-and-run programming of therapeutic cytoreagents using mRNA nanocarriers
Authors: HF Moffett, ME Coon, S Radtke, SB Stephan, L McKnight, A Lambert, BL Stoddard, HP Kiem, MT Stephan
Nat Commun, 2017-08-30;8(1):389.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling
Authors: Y Jin, L Muhl, M Burmakin, Y Wang, AC Duchez, C Betsholtz, HM Arthur, L Jakobsson
Nat. Cell Biol., 2017-05-22;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A Unique Cellular and Molecular Microenvironment Is Present in Tertiary Lymphoid Organs of Patients with Spontaneous Prostate Cancer Regression
Authors: ML García-Her, NO Uribe-Urib, R Espinosa-G, WM Kast, SA Khader, J Rangel-Mor
Front Immunol, 2017-05-17;8(0):563.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease
Sci Rep, 2016-09-23;6(0):32382.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Novel Rat Model of Repetitive Portal Venous Embolization Mimicking Human Non-Cirrhotic Idiopathic Portal Hypertension
PLoS ONE, 2016-09-02;11(9):e0162144.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Evaluation of Nonperfused Retinal Vessels in Ischemic Retinopathy
Invest Ophthalmol Vis Sci, 2016-09-01;57(11):5031-5037.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Authors: Riordan D, Varma S, West R, Brown P
PLoS ONE, 2015-07-15;10(7):e0128975.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients.
Authors: Martinez L, Labovsky V, Calcagno M, Davies K, Garcia Rivello H, Bianchi M, Wernicke A, Fernandez Vallone V, Chasseing N
PLoS ONE, 2015-03-24;10(3):e0121421.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Umbilical cord mesenchymal stromal cells affected by gestational diabetes mellitus display premature aging and mitochondrial dysfunction.
Authors: Kim J, Piao Y, Pak Y, Chung D, Han Y, Hong J, Jun E, Shim J, Choi J, Kim C
Stem Cells Dev, 2015-01-14;24(5):575-86.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Key role of the endothelial TGF-beta/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension.
Authors: Gore B, Izikki M, Mercier O, Dewachter L, Fadel E, Humbert M, Dartevelle P, Simonneau G, Naeije R, Lebrin F, Eddahibi S
PLoS ONE, 2014-06-23;9(6):e100310.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Expression of uPAR in tumor-associated stromal cells is associated with colorectal cancer patient prognosis: a TMA study.
Authors: Boonstra M, Verbeek F, Mazar A, Prevoo H, Kuppen P, van de Velde C, Vahrmeijer A, Sier C
BMC Cancer, 2014-04-17;14(0):269.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Glyceryl trinitrate inhibits hypoxia-induced release of soluble fms-like tyrosine kinase-1 and endoglin from placental tissues.
Authors: Barsoum IB, Renaud SJ, Graham CH
Am. J. Pathol., 2011-06-01;178(6):2888-96.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Glial cell line-derived neurotrophic factor-secreting genetically modified human bone marrow-derived mesenchymal stem cells promote recovery in a rat model of Parkinson's disease.
Authors: Glavaski-Joksimovic A, Virag T, Mangatu TA
J. Neurosci. Res., 2010-09-01;88(12):2669-81.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Cardiac Progenitor Cell–Derived Extracellular Vesicles Reduce Infarct Size and Associate with Increased Cardiovascular Cell Proliferation
Authors: Janita A. Maring, Kirsten Lodder, Emma Mol, Vera Verhage, Karien C. Wiesmeijer, Calinda K. E. Dingenouts et al.
Journal of Cardiovascular Translational Research
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Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells
Authors: Laura E. Sidney, Matthew J. Branch, Harminder S. Dua, Andrew Hopkinson
Cytotherapy
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Corneal keratocyte transition to mesenchymal stem cell phenotype and reversal using serum-free medium supplemented with fibroblast growth factor-2, transforming growth factor-beta 3 and retinoic acid
Authors: Laura E. Sidney, Andrew Hopkinson
Journal of Tissue Engineering and Regenerative Medicine
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Lysates and supernatants of pancreatic cancer cells AsPC-1 and MIA PaCa-2 were examined under denaturing/reducing conditions by SDS-PAGE/western blots and subsequent antibody stainings. Nitrocellulose membrane was probed with 0.13 ug/ml. This antibody stains endoglin as expected.