Human EMR3 Antibody

Catalog #: MAB8496 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8496
MAB8496-SP

Detection of EMR3 in Human Blood Monocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EMR3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EMR3 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 908235

Purification

Protein A or G purified from cell culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human EMR3
Met1-Tyr652
Accession # Q9BY15

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of human lung cancer tissue

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of EMR3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A) and either (A) Mouse Anti-Human EMR3 Monoclonal Antibody (Catalog # MAB8496) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Immunohistochemistry

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EMR3 in Human Lung Cancer Tissue. EMR3 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human EMR3 Monoclonal Antibody (Catalog # MAB8496) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMR3

EMR3 is a member of the class B seven-span transmembrane (TM7) receptor family. It is expressed predominantly by cells of the immune system, with highest levels in neutrophils, monocytes and macrophages. It may play a role in myeloid-myeloid interactions during immune and inflammatory responses. EMR3 upregulation in glioblastoma multiforme (GBM) is associated with poor survival and may be a potential therapeutic target.

Long Name

EGF-like Module Containing, Mucin-like, Hormone Receptor-like 3

Entrez Gene IDs

84658 (Human)

Alternate Names

egf-like module containing, mucin-like, hormone receptor-like 3; EGF-like module receptor 3; EGF-like module-containing mucin-like hormone receptor-like 3; egf-like module-containing mucin-like receptor 3; EMR3