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Human EGR1 Antibody

Catalog #: AF2818
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2818
AF2818-SP
Detection of EGR1 in SK‑BR‑3 Human Cell Line.
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Citations (13)
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Human EGR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGR1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human EGR1
Gln282-Ser433
Accession # P18146
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human EGR1
Immunocytochemistry
1.7-15 µg/mL
Immersion fixed SK-BR-3 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of EGR1 in SK‑BR‑3 Human Cell Line. EGR1 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line using Goat Anti-Human EGR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2818) at 5 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGR1

Early Growth Response 1, (EGR1) belongs to the EGR family of zinc finger transcription factors and regulates the expression of several tumor suppressors. Its expression is induced by diverse signals that initiate growth and differentiation. EGR1 suppresses or activates cell growth depending on the tumor type. Within the region that includes its three zinc finger domains, human EGR1 shares 99% aa sequence identity with mouse and rat EGR1.

Long Name
Early Growth Response 1
Entrez Gene IDs
1958 (Human)
Alternate Names
AT225Transcription factor ETR103; early growth response 1; early growth response protein 1; EGR1; EGR-1; G0S30; KROX24; KROX-24; Nerve growth factor-induced protein A; NGFIA; NGFI-ATranscription factor Zif268; TIS8; ZIF-268; Zinc finger protein Krox-24; ZNF225; ZNF225Zinc finger protein 225

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Citations for Human EGR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Wnt/ beta -catenin-signaling and the formation of Müller glia-derived progenitors in the chick retina
    Authors: Donika Gallina, Isabella Palazzo, Lillia Steffenson, Levi Todd, Andy J. Fischer
    Developmental Neurobiology
  2. Vision-guided ocular growth in a mutant chicken model with diminished visual acuity
    Authors: Eric R. Ritchey, Christopher Zelinka, Junhua Tang, Jun Liu, Kimberly A. Code, Simon Petersen-Jones et al.
    Experimental Eye Research
  3. High-frequency hearing in a hummingbird
    Authors: F. G. Duque, C. A. Rodriguez-Saltos, S. Uma, I. Nasir, M. F. Monteros, W. Wilczynski et al.
    Science Advances
  4. CD44 induced enhancement of phosphatase activity and calcium influx: Modifications of EGR-1 expression and cell proliferation
    Authors: Ronny R. Racine, Nathan A. Manalo, Jessica M.F. Hall, Adnan Dibas, Glen D. Raffel, Mark E. Mummert
    Biochemistry and Biophysics Reports
  5. A novel type of glial cell in the retina is stimulated by insulin-like growth factor 1 and may exacerbate damage to neurons and Muller glia.
    Authors: Fischer AJ, Scott MA, Zelinka C, Sherwood P
    Glia, 2010-04-15;58(6):633-49.
  6. Transcriptomic analysis reveals cell apoptotic signature modified by heparanase in melanoma cells
    Authors: T Song, D Spillmann
    J. Cell. Mol. Med., 2019-05-02;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Expression of early growth response-1 in colorectal cancer and its relation to tumor cell proliferation and apoptosis.
    Authors: Myung D, Park Y, Kim N, Chung C, Park H, Kim J, Cho S, Lee W, Lee J, Joo Y
    Oncol Rep, 2013-11-29;31(2):788-94.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Notch signaling influences neuroprotective and proliferative properties of mature Muller glia.
    Authors: Ghai K, Zelinka C, Fischer AJ
    J. Neurosci., 2010-02-24;30(8):3101-12.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  9. Mitogen-activated protein kinase-signaling regulates the ability of Muller glia to proliferate and protect retinal neurons against excitotoxicity.
    Authors: Fischer AJ, Scott MA, Ritchey ER, Sherwood P
    Glia, 2009-11-01;57(14):1538-52.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. Heparin-binding EGF-like growth factor (HB-EGF) stimulates the proliferation of Müller glia-derived progenitor cells in avian and murine retinas
    Authors: Levi Todd, Leo I. Volkov, Chris Zelinka, Natalie Squires, Andy J. Fischer
    Molecular and Cellular Neuroscience
  11. Jak/Stat signaling regulates the proliferation and neurogenic potential of Müller glia-derived progenitor cells in the avian retina
    Authors: Levi Todd, Natalie Squires, Lilianna Suarez, Andy J. Fischer
    Scientific Reports
  12. BMP- and TGF beta -signaling regulate the formation of Müller glia-derived progenitor cells in the avian retina
    Authors: Levi Todd, Isabella Palazzo, Natalie Squires, Ninoshka Mendonca, Andy J. Fischer
    Glia
  13. Reactive microglia and macrophage facilitate the formation of Müller glia-derived retinal progenitors
    Authors: Andy J. Fischer, Christopher Zelinka, Donika Gallina, Melissa A. Scott, Levi Todd
    Glia

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