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Human EGF Antibody

Catalog #: MAB236
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB236-SP
MAB236-100
MAB236-500
Detection of Recombinant Human and Rat EGF by Western Blot.
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Product Details
Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGF in direct ELISAs. Detects human EGF and rat EGF in Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) HB‑EGF or rhTGF‑ alpha is observed. In Western blots, no cross-reactivity with recombinant mouse EGF is observed.
Source
Monoclonal Mouse IgG1 Clone # 10825
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human EGF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.05-0.1 µg/mL in the presence of 2 ng/mL Recombinant Human EGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human and Rat EGF antibody by Western Blot. View Larger

Detection of Recombinant Human and Rat EGF by Western Blot. Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry EGF antibody in Human Skin by Immunohistochemistry (IHC-P). View Larger

EGF in Human Skin. EGF was detected in immersion fixed paraffin-embedded sections of human skin using 25 µg/mL Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes in the stratum granulosum in epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. View Larger

Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236). The ND50 is typically 0.05-0.1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF

EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Long Name
Epidermal Growth Factor
Entrez Gene IDs
1950 (Human); 13645 (Mouse); 25313 (Rat)
Alternate Names
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone

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Citations for Human EGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway
    Authors: Antonios Chronopoulos, Stephen D. Thorpe, Ernesto Cortes, Dariusz Lachowski, Alistair J. Rice, Vasyl V. Mykuliak et al.
    Nature Materials
  2. E6-mediated activation of JNK drives EGFR signalling to promote proliferation and viral oncoprotein expression in cervical cancer
    Authors: Ethan L. Morgan, James A. Scarth, Molly R. Patterson, Christopher W. Wasson, Georgia C. Hemingway, Diego Barba-Moreno et al.
    Cell Death & Differentiation
  3. Bisphenol S and Epidermal Growth Factor Receptor Signaling in Human Placental Cytotrophoblasts
    Authors: E Ticiani, J Gingrich, Y Pu, M Vettathu, J Davis, D Martin, MG Petroff, A Veiga-Lope
    Environmental health perspectives, 2021-02-19;129(2):27005.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Control
  4. VEGFR-1 Regulates EGF-R to Promote Proliferation in Colon Cancer Cells
    Authors: H Nagano, C Tomida, N Yamagishi, S Teshima-Ko
    Int J Mol Sci, 2019-11-09;20(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Gelatin-Alginate Complexes for EGF Encapsulation: Effects of H-Bonding and Electrostatic Interactions
    Authors: S Jeong, B Kim, HC Lau, A Kim
    Pharmaceutics, 2019-10-14;11(10):.
    Species: Human
    Sample Types: Coacervates
    Applications: Western Blot
  6. Case report of multiple pustules of the bilateral lower limbs caused by a granulocyte colony-stimulating factor-producing solid pseudopapillary tumour of the pancreas
    Authors: Y Iwata
    Br. J. Dermatol, 2017-08-20;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Epidermal growth factor as a mechanosensitizer in human bone marrow stromal cells
    Authors: S Müller-Deu, L Seefried, M Krug, F Jakob, R Ebert
    Stem Cell Res, 2017-08-18;24(0):69-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  8. Expression and clinical value of EGFR in human meningiomas
    Authors: MB Arnli, T Backer-Grø, B Ytterhus, US Granli, S Lydersen, S Gulati, SH Torp
    PeerJ, 2017-03-29;5(0):e3140.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype.
    Authors: Zhang Z, Dong Z, Lauxen I, Filho M, Nor J
    Cancer Res, 2014-03-31;74(10):2869-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Photodynamic therapy with 5-aminolevulinic acid (ALA) impairs tumor initiating and chemo-resistance property in head and neck cancer-derived cancer stem cells.
    Authors: Yu, Chuan-Ha, Yu, Cheng-Ch
    PLoS ONE, 2014-01-24;9(1):e87129.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
    Authors: Nadworny PL, Wang J, Tredget EE
    J Inflamm (Lond), 2010-02-19;7(0):13.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. S100A11, an dual mediator for growth regulation of human keratinocytes.
    Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
    Mol. Biol. Cell, 2007-10-31;19(1):78-85.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  13. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
    Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
    J. Invest. Dermatol., 2006-05-11;126(9):2096-105.
    Species: Human
    Sample Types: Serum
    Applications: Neutralization
  14. Salivary leptin induces increased expression of growth factors in oral keratinocytes.
    Authors: Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M
    J. Mol. Endocrinol., 2005-04-01;34(2):353-66.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptors
    Authors: Tamara Muliaditan, Leena Halim, Lynsey M. Whilding, Benjamin Draper, Daniela Y. Achkova, Fahima Kausar et al.
    Cell Reports Medicine

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