Human EDA2R/TNFRSF27/XEDAR Antibody

Catalog #: MAB10931 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10931
MAB10931-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EDA2R/TNFRSF27/XEDAR Antibody Summary

Species Reactivity

Human

Specificity

Detects human EDA2R/TNFRSF27/XEDAR in ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human (rh) 41BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR‑3, rhDR‑6, rhEDAR, rhFAS, rhGITR, rhHVEM, rhLTR‑b, rhNGFR, rhOPG, rhOX40 R, rhRANK, rhRELT, rhTAJ, rhTNF R1, rhTNF RII, rhTRADD, rhTRAIL R1, rhTRAIL R2, rhTRAIL R3, rhTRAIL R4, rhTWEAK R, recombinant mouse (rm) DcTRAIL R1, and rmDcTrail R2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 173103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EDA2R/TNFRSF27/XEDAR
Met1-Glu136
Accession # Q9HAV5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human EDA2R/TNFRSF27/XEDAR Fc Chimera (Catalog # 1093-XD)

Human EDA2R/TNFRSF27/XEDAR Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human EDA2R/TNFRSF27/XEDAR Biotinylated Antibody (Catalog # BAF1093)

Standard: Recombinant Human EDA2R/TNFRSF27/XEDAR Fc Chimera, CF (Catalog # 1093-XD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDA2R/TNFRSF27/XEDAR

X-linked Ectodysplasin Receptor (XEDAR), also known as EDA2R and TNFRSF27, is an approximately 45 kDa transmembrane protein in the TNF receptor superfamily (1). Mature human XEDAR consists of a 136 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain (2). Within the ECD, human XEDAR shares 87% aa sequence identity with mouse and rat XEDAR. A 55 kDa long isoform of human XEDAR carries a 21 aa insertion in the juxtamembrane cytoplasmic domain (3). A 20 kDa fragment of the ECD can be shed by metalloprotease mediated cleavage (4). XEDAR binds selectively to the EDA-A2 variant of Ectodysplasin (EDA), while the closely related receptor EDAR binds selectively to the EDA-A1 variant (2). Other than a 2 aa deletion in its TNF-like domain, EDA-A2 is identical to EDA-A1 (2). Mutations in both EDAR and EDA are associated with hypohidrotic ectodermal dysplasia (HED), a disorder of hair, tooth, and eccrine sweat gland morphogenesis (5). XEDAR itself is strongly associated with androgenetic alopecia (male hair loss) (6). XEDAR is widely expressed, notably in embryonic basal epidermal cells and maturing hair follicles (2, 7, 8). Even though it does not contain a cytoplasmic death domain, XEDAR can associate with Fas and induce EDA-A2 dependent apoptosis (7, 9). Its transcription is directly induced by p53, and XEDAR mediated cell death is p53 dependent (7, 10). XEDAR is down‑regulated in breast, colon, and lung cancers, particularly in cases with p53 mutations (7, 11). XEDAR also plays a role in EDA‑A2 induced skeletal muscle degeneration and osteoblast differentiation (8, 12).

References

Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
Yan, M. et al. (2000) Science 290:523.
Sinha, S.K. et al. (2002) J. Biol. Chem. 277:44953.
Tanikawa, C. et al. (2010) Mol. Cancer Res. 8:855.
Mikkola, M.L. (2009) Am. J. Med. Genet. 149A:2031.
Prodi, D.A. et al. (2008) J. Invest. Dermatol. 128:2268.
Tanikawa, C. et al. (2009) Oncogene 28:3081.
Newton, K. et al. (2004) Mol. Cell. Biol. 24:1608.
Sinha, S.K. and P.M. Chaudhary (2004) J. Biol. Chem. 279:41873.
Brosh, R. et al. (2010) FEBS Lett. 584:2473.
Punj, V. et al. (2010) Clin. Cancer Res. 16:1140.
Chang, B. et al. (2007) Cancer Gene Ther. 14:927.

Long Name

Ectodysplasin A2 Receptor

Entrez Gene IDs

60401 (Human); 245527 (Mouse); 710244 (Rhesus Macaque)

Alternate Names

ectodysplasin A2 receptor; EDA2R; EDA-A2 receptor; EDAA2R; EDA-A2R; EDAA2Rtumor necrosis factor receptor superfamily member XEDAR; EDAR2; TNFRSF27; XEDAR; XEDARTNFRSF27tumor necrosis factor receptor superfamily member 27; X-linked ectodysplasin-A2 receptor