Human E6AP/UBE3A Antibody Summary
Met1-Leu875
Accession # Q05086
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human E6AP/UBE3A by Western Blot. Western Blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11498) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E6AP/UBE3A at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human E6AP/UBE3A by Simple WesternTM. Simple Western shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/ml. A specific band was detected for E6AP/UBE3A at approximately 106 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11498). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E6AP/UBE3A
Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).
- Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343.
- Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156.
- Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095.
- Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765.
- Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407.
- Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.
Product Datasheets
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