Human E-Cadherin NorthernLights™ NL493-conjugated Antibody

Catalog #: NL648G Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
NL648G

All E-Cadherin Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human E-Cadherin NorthernLights™ NL493-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human E-Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse E-Cadherin is observed.

Source

Polyclonal Goat IgG

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

NL493 (Excitation= 493 nm, Emission= 514 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: E-Cadherin

Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E‑Cadherin may also play a role in tumor development, as loss of E‑Cadherin has been associated with tumor invasiveness. E‑Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.

References

Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
Overduin, M. et al. (1995) Science 267:386.
Takeichi, M. (1991) Science 251:1451.

Entrez Gene IDs

999 (Human); 12550 (Mouse); 83502 (Rat)

Alternate Names

Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin