Human DGK-beta Antibody

Catalog #: MAB6727 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6727
MAB6727-SP

Detection of Human DGK‑ beta by Western Blot.

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All DGK-beta Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DGK-beta Antibody Summary

Species Reactivity

Human

Specificity

Detects human DGK-beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DGK-alpha, gamma, epsilon, iota, kappa, theta, or zeta is observed.

Source

Monoclonal Mouse IgG₁ Clone # 646523

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DGK-beta
Met1-Ser143
Accession # Q9Y6T7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DGK‑ beta by Western Blot. Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DGK-beta Monoclonal Antibody (Catalog # MAB6727) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-beta at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGK-beta

Diacylglycerol kinase beta (DGK-beta ) is an approximately 90 kDa kinase that phosphorylates the lipid diacylglycerol. It is expressed in the brain and colocalizes with D1 and D2 dopamine receptors. DGK-beta promotes the formation and maturation of dendritic spines. It contains two EF-hand motifs (aa 149-184 and 194-229), two zinc finger regions (aa 244-294 and aa 309-358), and a catalytic domain (aa 434-568). Alternate splicing generates an additional isoform with a 31 aa C-terminal deletion. Over aa 1-143, human DGK-beta shares 97% aa sequence identity with mouse and rat DGK-beta.

Long Name

Diacylglycerol Kinase, beta 90kDa

Entrez Gene IDs

1607 (Human); 217480 (Mouse); 54248 (Rat)

Alternate Names

90 kDa diacylglycerol kinase; DAG kinase beta; DAGK2; DGKB; DGKbeta; DGK-beta; diacylglycerol kinase beta; diacylglycerol kinase, beta (90kD); diacylglycerol kinase, beta 90kDa; Diglyceride kinase beta; EC 2.7.1; EC 2.7.1.107; KIAA0718DGKDGK-BETA