Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody

Catalog #: AF5826 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5826
AF5826-SP

Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 by Western Blot.

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All Cytosolic Sulfotransferase 4A1/SULT4A1 Antibodies

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Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SULT4A1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SULT1A1, rhSULT2A1, and rhSULT1E1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SULT4A1 isoform 1
Ala2-Leu284
Accession # Q9BR01

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Cell lysates spiked with Recombinant Human Cytosolic Sulfotransferase 4A1/SULT4A1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 by Western Blot. Western blot shows lysates of human brain cortex and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5826) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 4A1/SULT4A1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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SULT4A1 in Human Brain SULT4A1 was detected in paraffin-embedded sections of human brain (cortex) using Goat Anti-Human SULT4A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5826) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 4A1/SULT4A1

SULT4A1 (Cytosolic Sulfotransferase 4A1; also BR-STL1/Brain sulfotransferase-like protein) is a 33-36 kDa member of the sulfotransferase 4 family of enzymes. SULT4A1 message is widely expressed. However, it appears to be translated only in brain. SULT4A1 is very unusual in that it is the only SULT that shows minimal activity on synthetic laboratory substrates. Instead, it acts on catecholamines and T4 in a manner that may not involve sulfonation. Human SULT4A1 is 284 amino acids (aa) in length. A weak PAPS binding site is found between aa 246-254. There are two potential isoforms; one that shows a 13 aa substitution for aa 248-284 and a second that contains a 48 aa substitution for aa 202 - 247. Full-length human SULT4A1 is 98% aa identical to mouse SULT4A1.

Entrez Gene IDs

25830 (Human); 29859 (Mouse)

Alternate Names

Brain sulfotransferase-like protein; BRSTL1; BR-STL-1; Cytosolic Sulfotransferase 4A1; EC 2.8.2.-; hBR-STL; hBR-STL-1MGC40032; nervous system cytosolic sulfotransferase; Nervous system sulfotransferase; NST; ST4A1; sulfotransferase 4A1; sulfotransferase family 4A, member 1; sulfotransferase-related protein; SULT4A1; SULTX3; SULTX3DJ388M5.3