Human CXCR2/IL-8 RB PerCP-conjugated Antibody

Name: Human CXCR2/IL-8 RB PerCP-conjugated Antibody
Brand: R&D Systems
SKU: FAB331C

Catalog #: FAB331C Datasheet / COA / SDS

Clone 48311 was used by HLDA to establish CD designation

Discontinued Product

FAB331C has been discontinued.

View all CXCR2/IL-8RB products.

Detection of CXCR2/IL‑8 RB in Human Blood Neutrophils by Flow Cytometry.

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All CXCR2/IL-8RB Antibodies

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Human CXCR2/IL-8 RB PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).

Source

Monoclonal Mouse IgG_2A Clone # 48311

Purification

Protein A or G purified from ascites

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR2/IL-8 RB
Met1-Leu355
Accession # AAB25880

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR2/IL‑8 RB in Human Blood Neutrophils by Flow Cytometry. Human peripheral blood neutrophils were stained with Mouse Anti-Human CXCR2/IL-8 RB PerCP-conjugated Monoclonal Antibody (Catalog # FAB331C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR2/IL-8RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

Long Name

Interleukin 8 Receptor B

Entrez Gene IDs

3579 (Human); 12765 (Mouse); 29385 (Rat)

Alternate Names

CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; CMKAR2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 RB; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B