Human CXCL14/BRAK Biotinylated Antibody

Catalog #: BAM8661 Datasheet / COA / SDS

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BAM8661

All CXCL14/BRAK Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCL14/BRAK Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCL14/BRAK in ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 131107

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CXCL14/BRAK
Ser35-Glu111
Accession # O95715

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Human CXCL14/BRAK Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human CXCL14/BRAK Antibody (Catalog # MAB866)

Standard: Recombinant Human CXCL14/BRAK Protein, CF (Catalog # 866-CX)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL14/BRAK

CXCL14/BRAK (breast and kidney-expressed chemokine), also named MIP-2 gamma, KEC (kidney-expressed chemokine), and BMAC (B cell and monocyte-activating chemokine), is a member of CXC chemokine superfamily (1 - 5). The deduced 99 amino acid (aa) residue precursor has a 22 aa putative signal peptide that is cleaved to produce the 77 aa mature protein. Mature human and mouse CXCL14 differ by only 2 residues. Human CXCL14 shares approximately 30% aa sequence identity with MIP-2 alpha (GRO beta ) as well as MIP-2 beta (GRO gamma ). The gene for CXCL14 has been mapped human chromosome 5q31. Unlike the MIP-2 chemokines, CXCL14 lacks the ELR domain preceding the CXC motif. CXCL14 transcripts are constitutively expressed at high levels in the basal layer of epidermal keratinocytes and dermal fibroblasts of skin tissues as well as lamina propria cells in normal intestinal tissues. CXCL14 has been shown to be a highly selective chemoattractant for monocytes that have been treated with prostaglandin E₂ or forskolin, agents that activate adenylate cyclase. CXCL14 has been proposed to be important for regulating the trafficking of macrophage precursor to regions in skin and mucosal tissues that support their development. Consistent with this hypothesis, macrophages were frequently found to co-localize with CXCL14-producing cells in the dermis and lamina propria.

References

Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 255:703.
Cao, X. et al. (2000) J. Immunol. 165:2588.
Kurth, I. et al. (2001) J. Exp., Med. 194:855.
Frederick, M.J. et al. (2000) Am. J. Pathol. 156:1937.
Sleeman, M.A. et al. (2000) Int. Immunol. 12:677.

Entrez Gene IDs

9547 (Human); 57266 (Mouse)

Alternate Names

BMAC; Bolekine; BRAK; BRAKKEC; chemokine (C-X-C motif) ligand 14; Chemokine BRAK; CXCL14; CXC-X3; member 14 (BRAK); MIP-2 gamma; MIP2G; MIP-2G; NJACKec; SCYB14MGC10687; Small-inducible cytokine B14