Human CXADR Biotinylated Antibody

Catalog #: BAF3336 Datasheet / COA / SDS

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BAF3336

All CXADR Antibodies

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Human CXADR Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXADR in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse CXADR is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CXADR
Leu20-Gly237
Accession # P78310

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CXADR Fc Chimera (Catalog # 3336-CX)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXADR

CXADR (coxsackie and adenovirus receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1‑3). CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CXADR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4‑6). The 365 amino acid (aa) human CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). The C‑terminus interacts with several cytoplasmic junctional proteins, microtubules and the actin cytoskeleton (1, 8, 9). The ECD of human CXADR shares 90% aa sequence identity with mouse, rat, and porcine CXADR, and 92% and 89% aa identity with bovine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C‑terminal 15 aa shows a similar expression pattern (4, 10). Transcription of splice variants that produce soluble forms of CXADR has been detected, and secreted forms in serum and pleural fluid potentially block viral infection (11).

References

Coyne, C.B. and J.M. Bergelson (2005) Adv. Drug Deliv. Rev. 57:869.
Philipson, L. and R.F. Pettersson (2004) Curr. Top. Microbiol. Immunol. 273:87.
Tomko, R.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3352.
Raschperger, E. et al. (2006) Exp. Cell Res. 312:1566.
Hotta, Y. et al. Dev. Brain Res. 143:1.
Hauwel, M. et al. (2005) Brain Res. Rev. 48:265.
Chen, J. et al. (2006) Circ. Res. 98:923.
Fok, P.T. et al. (2007) J. Biol. Chem. 282:7512.
Huang, K-C. et al. (2007) FEBS Lett. 581:2702.
Mirza, M. et al. (2006) Exp. Cell Res. 312:817.
Bernal, R.M. et al. (2002) Clin. Cancer Res. 8:1915.

Long Name

Coxsackie and Adenovirus Receptor

Entrez Gene IDs

1525 (Human); 13052 (Mouse)

Alternate Names

CAR10Coxsackievirus B-adenovirus receptor; CAR4/6; coxsackie virus and adenovirus receptor; coxsackie virus B receptor; coxsackievirus and adenovirus receptor; CVB3 binding protein; CVB3 BP; CVB3-binding protein; CXADR; HCAR; HCVADR