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Human CXADR Antibody

Catalog #: AF3336
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3336
AF3336-SP
Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXADR Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXADR in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse CXADR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CXADR
Leu20-Gly237
Accession # P78310
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CXADR Fc Chimera (Catalog # 3336-CX)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXADR

CXADR (coxsackie and adenovirus receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1‑3). CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CXADR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4‑6). The 365 amino acid (aa) human CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). The C‑terminus interacts with several cytoplasmic junctional proteins, microtubules and the actin cytoskeleton (1, 8, 9). The ECD of human CXADR shares 90% aa sequence identity with mouse, rat, and porcine CXADR, and 92% and 89% aa identity with bovine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C‑terminal 15 aa shows a similar expression pattern (4, 10). Transcription of splice variants that produce soluble forms of CXADR has been detected, and secreted forms in serum and pleural fluid potentially block viral infection (11).

References
  1. Coyne, C.B. and J.M. Bergelson (2005) Adv. Drug Deliv. Rev. 57:869.
  2. Philipson, L. and R.F. Pettersson (2004) Curr. Top. Microbiol. Immunol. 273:87.
  3. Tomko, R.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3352.
  4. Raschperger, E. et al. (2006) Exp. Cell Res. 312:1566.
  5. Hotta, Y. et al. Dev. Brain Res. 143:1.
  6. Hauwel, M. et al. (2005) Brain Res. Rev. 48:265.
  7. Chen, J. et al. (2006) Circ. Res. 98:923.
  8. Fok, P.T. et al. (2007) J. Biol. Chem. 282:7512.
  9. Huang, K-C. et al. (2007) FEBS Lett. 581:2702.
  10. Mirza, M. et al. (2006) Exp. Cell Res. 312:817.
  11. Bernal, R.M. et al. (2002) Clin. Cancer Res. 8:1915.
Long Name
Coxsackie and Adenovirus Receptor
Entrez Gene IDs
1525 (Human); 13052 (Mouse)
Alternate Names
CAR10Coxsackievirus B-adenovirus receptor; CAR4/6; coxsackie virus and adenovirus receptor; coxsackie virus B receptor; coxsackievirus and adenovirus receptor; CVB3 binding protein; CVB3 BP; CVB3-binding protein; CXADR; HCAR; HCVADR

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Citations for Human CXADR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Construction of a pIX-modified Adenovirus Vector Able to Effectively Bind to Nanoantibodies for Targeting
    Authors: M. N. Garas, S. V. Tillib, O. V. Zubkova, V. N. Rogozhin, T. I. Ivanova, L. A. Vasilev et al.
    Acta Naturae
  2. Exogenous LRRK2G2019S induces parkinsonian-like pathology in a nonhuman primate
    Authors: N Mestre-Fra, N Serratrice, A Gennetier, G Devau, S Cobo, SG Trouche, P Fontès, C Zussy, P De Deurwae, S Salinas, FJ Mennechet, J Dusonchet, BL Schneider, I Saggio, V Kalatzis, MR Luquin-Piu, JM Verdier, EJ Kremer
    JCI Insight, 2018-07-26;3(14):.
    Species: Primate
    Sample Types: Whole Tissue
    Applications: IHC
  3. Induction of the Coxsackievirus and Adenovirus Receptor in Macrophages During the Formation of Atherosclerotic Plaques
    Authors: Azadeh Nilchian, Estelle Plant, Malgorzata M Parniewska, Ana Santiago, Aránzazu Rossignoli, Josefin Skogsberg et al.
    The Journal of Infectious Diseases

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