Human CX3CR1 PE-conjugated Antibody

Catalog #: FAB5204P
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB5204P-025
FAB5204P-100

Detection of CX3CR1 in Human Blood Monocytes by Flow Cytometry.

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All CX3CR1 Antibodies

Product Details

Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CX3CR1 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CX3CR1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 528728

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CX3CR1
Met1-Thr31, Leu91-Lys103, Thr168-Thr195, Lys257-Leu273
Accession # NP_001328

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CX3CR1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CX3CR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5204P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CX3CR1 in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Mouse Anti-Human CX3CR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5204P) or (B) Mouse IgG1 isotype control antibody (Catalog # IC002P) and Mouse anti-Human CD14 APC-conjugated antibody (Catalog # FAB3832A). Staining was done at 37^oC. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CX3CR1

CX3CR1 is a 40 kDa seven transmembrane chemokine receptor that is expressed on several T cell subsets, monocytes, macrophages, microglia, and epithelial cells. CX3CR1 binding to membrane bound or soluble CX3CR1/Fractalkine promotes inflammatory responses by inducing monocyte adhesion to endothelial cells and macrophage activation. CX3CR1 polymorphisms are associated with the development of chronic inflammatory disorders. Alternately spliced isoforms have extended N-terminal extracellular regions that increase the potency of CX3CR1 as a fusion co-receptor for HIV-1. Human CX3CR1 shares 82% amino acid sequence identity with mouse and rat CX3CR1.

Long Name

CX3C Chemokine Receptor 1

Entrez Gene IDs

1524 (Human); 13051 (Mouse); 171056 (Rat)

Alternate Names

Beta chemokine receptor-like 1; CCRL1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMKbRL1; CMK-BRL1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3CR1; Fractalkine receptor; G protein-coupled receptor 13; GPR13G-protein coupled receptor 13; GPRV28; V28; V28C-X3-C CKR-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CX3CR1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells.
Authors: Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B
iScience, 2021-11-14;24(12):103433.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial
Authors: S Sbrana, J Campolo, A Clemente, L Bastiani, A Cecchettin, E Ceccherini, C Caselli, D Neglia, O Parodi, D Chiappino, JM Smit, AJ Scholte, G Pelosi, S Rocchiccio
Biomed Res Int, 2020-07-27;2020(0):8748934.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Phenotypic changes of peripheral blood mononuclear cells upon corticosteroid treatment in idiopathic intermediate uveitis
Authors: Karoline Walscheid
Clin. Immunol, 2016-10-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
CX3CL1/CX3CR1 and CCL2/CCR2 Chemokine/Chemokine Receptor Complex in Patients with AMD.
Authors: Falk M, Singh A, Faber C, Nissen M, Hviid T, Sorensen T
PLoS ONE, 2014-12-15;9(12):e112473.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
Authors: Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido
J Immunol, 2014-03-19;192(8):3805-15.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Changes of peripheral TGF-beta1 depend on monocytes-derived macrophages in Huntington disease.
Authors: Di Pardo A, Alberti S, Maglione V, Amico E, Cortes E, Elifani F, Battaglia G, Busceti C, Nicoletti F, Vonsattel J, Squitieri F
Mol Brain, 2013-12-13;6(0):55.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry