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Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

Catalog #: BAM365
5 Citations Datasheet / COA / SDS
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BAM365
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Citations (5)
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Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CX3CL1 in ELISAs and Western blots.  In ELISAs, this antibody shows approximately 8% cross-reactivity with recombinant mouse (rm) CXCL6 and less than 0.2% cross-reactivity with rhCXCL6, rhCXCL9, and rrCX3CL1.
Source
Monoclonal Mouse IgG1 Clone # 51637
Purification
Protein A or G purified from ascites
Immunogen
NS0-derived and E.coli-derived Recombinant Human Fractalkine
Gln25-Arg339
Accession # P78423
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CX3CL1/Fractalkine Full Length (Catalog # 365-FR)

Human CX3CL1/Fractalkine Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # MAB3652)

Standard: Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (Catalog # 365-FR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

Fractalkine is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Entrez Gene IDs
6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names
ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

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Citations for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion
    Authors: A Babendreye, L Molls, D Dreymuelle, S Uhlig, A Ludwig
    Mediators Inflamm., 2017-03-26;2017(0):1515389.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  2. Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis
    Authors: N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton
    Arthritis Res. Ther, 2017-03-07;19(1):45.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  3. Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration.
    Authors: Popovic M, Laumonnier Y, Burysek L, Syrovets T, Simmet T
    J. Leukoc. Biol., 2008-04-24;84(1):215-23.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  4. Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes.
    Authors: Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A
    J. Immunol., 2007-06-15;178(12):8064-72.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  5. Fractalkine mediates natural killer-dependent antitumor responses in vivo.
    Authors: Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao JL, Maho M, Boissonnas A, Murphy PM, Debre P, Combadiere C
    Cancer Res., 2003-11-01;63(21):7468-74.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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