Human CRHBP Antibody Summary
Tyr25-Leu322
Accession # P24387.2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CRHBP in Human Liver Cancer. CRHBP was detected in immersion fixed paraffin-embedded sections of Human Liver Cancer using Goat Anti-Human CRHBP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2796) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRHBP
Human CRHBP is a 37 kDa secreted glycoprotein that likely serves to regulate CRH availability to the adrenal. In human, CRHBP complexes with CRH to form a circulating 41 kDa complex. In rodent and horse, this complex is restricted to the CNS. Human CRHBP is synthesized as a 322 amino acid (aa) precursor that contains a 24 aa signal sequence and a 298 aa mature region. The mature region contains one potential CUB domain and five intrachain disulfide bonds. Human mature CRHBP shares 87% and 84% sequence identity with mouse and equine CRHBP, respectively.
Product Datasheets
Citations for Human CRHBP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Decreased CRHBP expression is predictive of poor prognosis in patients with hepatocellular carcinoma
Authors: HB Xia, HJ Wang, LQ Fu, SB Wang, L Li, GQ Ru, XL He, XM Tong, XZ Mou, DS Huang
Oncol Lett, 2018-07-04;16(3):3681-3689.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Reduced mrna expression level of corticotropin-releasing hormone-binding protein is associated with aggressive human kidney cancer.
Authors: Tezval H, Atschekzei F, Peters I, Waalkes S, Hennenlotter J, Stenzl A, Becker J, Merseburger A, Kuczyk M, Serth J
BMC Cancer, 2013-04-22;13(0):199.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot
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