Human Complement Factor H-related 5/CFHR5 Biotinylated Antibody

Catalog #: BAF3845 Datasheet / COA / SDS

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BAF3845

All Complement Factor H-related 5/CFHR5 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Factor H-related 5/CFHR5 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement Factor H-related 5/CFHR5 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Factor H-related 5/CFHR5
Glu19-Glu569
Accession # Q9BXR6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Complement Factor H-related 5/CFHR5 (Catalog # 3845-F5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor H-related 5/CFHR5

The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H‑like protein 1 (FHL-1) and five factor H‑related proteins (FHR-1 to -5) (2). Members of this family are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. The genes of this family have been located in human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (3). FHR-5 has been identified initially as a universal component of complement deposits (1), and detected in glomerular immune deposits (4). The pattern of deposits is similar to other complement components, suggesting that FHR-5 may play a role in complement activation and regulation. It is synthesized in the liver and consists of 9 SCRs. Its biological function is not understood fully. FHR-5 exhibits similar characteristics as those of factor H in heparin binding, CRP binding, and lipoprotein association (5). Weak factor I-dependent cofactor activity for C3b cleavage has also been observed (5).

References

McRae, J. L. et al. (2001) J. Biol. Chem. 276:6747.
Zipfel, P. F. et al. (2002) Biochem. Soc. Trans. 30:971.
McRae, J. L. et al. (2002) Genetica. 114:157.
Murphy, B. et al. (2002) Am. J. Kidney. Dis. 39:24.
McRae, J. L. et al. (2005) J. Immunol. 174:6250.

Entrez Gene IDs

81494 (Human)

Alternate Names

CFHL5; CFHL5factor H-related protein 5; CFHR5; Complement Factor Hrelated 5; Complement Factor H-related 5; FHR5; FHR-5complement factor H-related protein 5; FHR5MGC133240; FLJ10549