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Human Complement Component C5aR1 Antibody

Catalog #: MAB3648
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3648
MAB3648-SP
N-Acetyl-beta -D-Glucosa-minidase Release Induced by Complement Component C5a and Neutralization by Human Complement Component C5a R1 Antibody.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Component C5aR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Complement Component C5a R1. Stains human Complement Component C5a R1 transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 347214
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Complement Component C5a R1
Met1-Val350
Accession # P21730
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood granulocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize Complement Component C5a-induced N-acetyl-beta -D-glucosaminidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Human Complement Component C5a and cytochalasin‑B.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization N-Acetyl-beta -D-Glucosa-minidase Release Induced by Complement Component C5a and Neutralization by Human Complement Component C5a R1 Antibody. View Larger

N-Acetyl-beta -D-Glucosa-minidase Release Induced by Complement Component C5a and Neutralization by Human Complement Component C5a R1 Antibody. Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N-acetyl-beta -D-glucosa-minidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta -D-Glucosaminidase Release elicited by Recombinant Human Complement Component C5a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Complement Component C5a R1 Monoclonal Antibody (Catalog # MAB3648). The ND50 is typically 2-10 µg/mL in the presence of cytochalasin-B.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C5aR1

Complement Component C5a R1 (C5aR1), also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5aR binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5aR1/C5a interactions are protective during allergen sensitization. Human C5aR1 shares 66% amino acid sequence identity with mouse and rat C5aR.

Long Name
Complement Component C5a Receptor 1
Entrez Gene IDs
728 (Human); 12273 (Mouse)
Alternate Names
C5a anaphylatoxin chemotactic receptor; C5A; C5aR; C5a-R; C5AR1; C5ARC5a anaphylatoxin receptor; C5R1C5a ligand; CD88 antigen; CD88; complement component 5 receptor 1 (C5a ligand); complement component 5 receptor 1; complement component 5a receptor 1; Complement Component C5a R1; Complement Component C5aR1

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Citations for Human Complement Component C5aR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Host-Receptor Post-Translational Modifications Refine Staphylococcal Leukocidin Cytotoxicity
    Authors: AT Tromp, M Van Gent, JP Jansen, LM Scheepmake, A Velthuizen, CJC De Haas, KPM Van Kessel, BW Bardoel, M Boettcher, MT McManus, JAG Van Strijp, RJ Lebbink, PA Haas, AN Spaan
    Toxins (Basel), 2020-02-06;12(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. C5a and C5aR are elevated in joints of rheumatoid and psoriatic arthritis patients, and C5aR blockade attenuates leukocyte migration to synovial fluid.
    Authors: Hornum L, Hansen A, Tornehave D, Fjording M, Colmenero P, Watjen I, Soe Nielsen N, Bliddal H, Bartels E
    PLoS ONE, 2017-12-08;12(12):e0189017.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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