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Human COMP/Thrombospondin-5 Antibody

Catalog #: AF3134
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3134
AF3134-SP
Detection of Human COMP/Thrombospondin‑5 by Western Blot.
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Citations (3)
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Human COMP/Thrombospondin-5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human COMP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse COMP is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human COMP/Thrombospondin-5
Gln21-Ala757 (Ala256Arg)
Accession # P49747
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
See below
Neutralization
Measured by its ability to neutralize COMP/Thrombospondin‑5-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 10 µg/mL Recombinant Human COMP/Thrombospondin‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human COMP/Thrombospondin-5 antibody by Western Blot. View Larger

Detection of Human COMP/Thrombospondin‑5 by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Human COMP/Thrombospondin-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for COMP/Thrombospondin-5 at approximately 90 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization Cell Adhesion Mediated by COMP/Thrombospondin‑5 and Neutralization by Human COMP/Thrombospondin‑5 Antibody. View Larger

Cell Adhesion Mediated by COMP/Thrombospondin‑5 and Neutralization by Human COMP/Thrombospondin‑5 Antibody. Recombinant Human COMP/Thrombospondin-5 (Catalog # 3134-CP), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human COMP/Thrombospondin-5 (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human COMP/Thrombo-spondin-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134). The ND50 is typically 1-4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COMP/Thrombospondin-5

Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). The human COMP cDNA encodes a 757 amino acid (aa) precursor that includes a 20 aa signal sequence followed by a non-collagenous
coiled‑coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain (5). Human COMP shares 86-93% aa sequence identity with rat, mouse, equine, bovine, and canine COMP. Within the TSP type-3 repeats and TSP C-terminal domain, human COMP shares 60%, 61%, 74%, and 80% aa sequence identity with human Thrombospondin-1, -2, -3, and -4, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alpha V beta 3 (9). COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type-3 repeat, results in diminished calcium binding ability (13, 14).

References
  1. Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961.
  2. Posey, K.L. et al. (2004) Int. J. Biochem. Cell Biol. 36:1005.
  3. Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102.
  4. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
  5. Newton, G. et al. (1994) Genomics, 24:435.
  6. DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422.
  7. Efimov, V.P. et al. (1994) FEBS Lett. 341:54.
  8. Ozbek, S. et al. (2002) EMBO J. 21:5960.
  9. Chen, F.H. et al. (2005) J. Biol. Chem. 280:32655.
  10. Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278.
  11. Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296.
  12. Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207.
  13. Kennedy, J. et al. (2005) Eur. J. Hum. Genet. 13:547.
  14. Hou, J. et al. (2000) Cell Calcium 27:309.
Long Name
Cartilage Oligomeric Matrix Protein
Entrez Gene IDs
1311 (Human); 12845 (Mouse)
Alternate Names
cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5

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Citations for Human COMP/Thrombospondin-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Intracellular mechanisms of molecular recognition and sorting for transport of large extracellular matrix molecules
    Proc Natl Acad Sci USA, 2016-09-27;0(0):.
  2. Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP
    Authors: L Sun, Y Wang, L Wang, B Yao, T Chen, Q Li, Z Liu, R Liu, Y Niu, T Song, Q Liu, K Tu
    J. Exp. Clin. Cancer Res., 2019-04-18;38(1):170.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Cartilage oligomeric matrix protein is differentially expressed in human subcutaneous adipose tissue and regulates adipogenesis
    Authors: N Denton, KE Pinnick, F Karpe
    Mol Metab, 2018-07-27;16(0):172-179.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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