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Human CLEC4D/CLECSF8 Antibody

Catalog #: MAB2806
2 Citations Datasheet / COA / SDS
Clone 413512 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB2806
MAB2806-SP
Detection of CLEC4D/CLECSF8 in Human Monocytes by Flow Cytometry.
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Citations (2)
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Human CLEC4D/CLECSF8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC4D/CLECSF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CLECSF9, rhCLECSF13, rhOCIL, or rhOCILrp2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 413512
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC4D/CLECSF8
Gly52-Asn215
Accession # Q8WXI8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CLEC4D/CLECSF8 under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CLEC4D/CLECSF8 antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of CLEC4D/CLECSF8 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human CLEC4D/CLECSF8 Monoclonal Antibody (Catalog # MAB2806, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC4D/CLECSF8

CLEC4D (C-type lectin domain family 4 member D), also known as CLCSF8, CLEC-6, and MCL, is a 30 kDa type II transmembrane (TM) glycoprotein that belongs to the CLR (C-type Lectin Receptor) family of molecules. It is synthesized as a 215 amino acid (aa) protein that contains a 17 aa N-terminal cytoplasmic domain, a 21 aa TM segment, and a 177 aa C-terminal extracellular region. The extracellular region shows a short stalk and a 118 aa CRD (carbohydrate recognition domain). The nature of its carbohydrate ligand is unknown. CLEC4D is restricted to monocytes/macrophages and serves as an endocytic receptor. Homodimers and homotrimers form on the cell surface. The human CLEC4D extracellular region shares 63% aa sequence identity with the mouse extracellular region.

Long Name
C-type Lectin Domain Family 4, Member D
Entrez Gene IDs
338339 (Human); 17474 (Mouse)
Alternate Names
CD368; CLEC4D; CLEC6; CLEC-6; CLECSF8; CLECSF8Mpcl; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 8; C-type lectin domain family 4 member D; C-type lectin domain family 4, member D; C-type lectin receptor; C-type lectin superfamily member 8; C-type lectin-like receptor 6; macrophage C-type lectin; MCL; MGC40078; Mpcl

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Citations for Human CLEC4D/CLECSF8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CD32a is a marker of a CD4 T-cell HIV reservoir harbouring replication-competent proviruses
    Authors: B Descours, G Petitjean, JL López-Zara, T Bruel, R Raffel, C Psomas, J Reynes, C Lacabaratz, Y Levy, O Schwartz, JD Lelievre, M Benkirane
    Nature, 2017-03-15;543(7646):564-567.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The C-type Lectin Receptor CLECSF8 (CLEC4D) Is Expressed by Myeloid Cells and Triggers Cellular Activation through Syk Kinase.
    Authors: Graham LM, Gupta V, Schafer G, Reid DM, Kimberg M, Dennehy KM, Hornsell WG, Guler R, Campanero-Rhodes MA, Palma AS, Feizi T, Kim SK, Sobieszczuk P, Willment JA, Brown GD
    J. Biol. Chem., 2012-06-11;287(31):25964-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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